Circulating lymphocytes and monocytes transcriptomic analysis of patients with type 2 diabetes mellitus, dyslipidemia and periodontitis

Autor: Bárbara Roque da Silva, Alliny de Souza Bastos, Catarina Satie Takahashi, Diego Girotto Bussaneli, Cláudia Vianna Maurer-Morelli, Silvana Regina Perez Orrico, Raquel M. Scarel-Caminaga, Silvana P. Barros, Sâmia Cruz Tfaile Corbi, Benilton S. Carvalho, Jaira F. de Vasconcellos, Cristiane S. Rocha, Raquel Alves dos Santos
Přispěvatelé: Universidade Estadual Paulista (Unesp), Room 9D11, Uniformed Services University of the Health Sciences and Henry Jackson Foundation for the Advancement of Military Medicine, Postgraduate Program in Sciences of the University of Franca, Universidade de São Paulo (USP), Universidade Estadual de Campinas (UNICAMP), School of Dentistry, Union of the Colleges of the Great Lakes (UNILAGO)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Scientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
Scientific Reports
Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 2045-2322
Popis: Made available in DSpace on 2020-12-12T02:06:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-12-01 Type 2 diabetes mellitus (T2DM), dyslipidemia and periodontitis are frequently associated pathologies; however, there are no studies showing the peripheral blood transcript profile of these combined diseases. Here we identified the differentially expressed genes (DEGs) of circulating lymphocytes and monocytes to reveal potential biomarkers that may be used as molecular targets for future diagnosis of each combination of these pathologies (compared to healthy patients) and give insights into the underlying molecular mechanisms of these diseases. Study participants (n = 150) were divided into groups: (H) systemically and periodontal healthy (control group); (P) with periodontitis, but systemically healthy; (DL-P) with dyslipidemia and periodontitis; (T2DMwell-DL-P) well-controlled type 2 diabetes mellitus with dyslipidemia and periodontitis; and (T2DMpoorly-DL-P) poorly-controlled type 2 diabetes mellitus with dyslipidemia and periodontitis. We preprocessed the microarray data using the Robust Multichip Average (RMA) strategy, followed by the RankProd method to identify candidates for DEGs. Furthermore, we performed functional enrichment analysis using Ingenuity Pathway Analysis and Gene Set Enrichment Analysis. DEGs were submitted to pairwise comparisons, and selected DEGs were validated by quantitative polymerase chain reaction. Validated DEGs verified from T2DMpoorly-DL-P versus H were: TGFB1I1, VNN1, HLADRB4 and CXCL8; T2DMwell-DL-P versus H: FN1, BPTF and PDE3B; DL-P versus H: DAB2, CD47 and HLADRB4; P versus H: IGHDL-P, ITGB2 and HLADRB4. In conclusion, we identified that circulating lymphocytes and monocytes of individuals simultaneously affected by T2DM, dyslipidemia and periodontitis, showed an altered molecular profile mainly associated to inflammatory response, immune cell trafficking, and infectious disease pathways. Altogether, these results shed light on novel potential targets for future diagnosis, monitoring or development of targeted therapies for patients sharing these conditions. Department of Diagnosis and Surgery School of Dentistry at Araraquara UNESP- São Paulo State University Department of Morphology Genetics Orthodontics and Pediatric Dentistry School of Dentistry at Araraquara UNESP- São Paulo State University Molecular Genomics and Therapeutics Section Genetics of Development and Disease Branch National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health 10 Center Drive Building 10 Room 9D11 Department of Surgery Uniformed Services University of the Health Sciences and Henry Jackson Foundation for the Advancement of Military Medicine Postgraduate Program in Sciences of the University of Franca Department of Genetics Faculty of Medicine of Ribeirão Preto USP – University of São Paulo Department of Biology Faculty of Philosophy Sciences and Letters of Ribeirão Preto USP –University of São Paulo Department of Medical Genetics and Medicine Genomics University of Campinas – UNICAMP Department of Statistics Institute of Mathematics Statistics and Scientific Computing University of Campinas Department of Periodontology University of North Carolina at Chapel Hill – UNC School of Dentistry Advanced Research Center in Medicine Union of the Colleges of the Great Lakes (UNILAGO) Department of Diagnosis and Surgery School of Dentistry at Araraquara UNESP- São Paulo State University Department of Morphology Genetics Orthodontics and Pediatric Dentistry School of Dentistry at Araraquara UNESP- São Paulo State University
Databáze: OpenAIRE
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