Neuropilin-2 is a novel marker expressed in pancreatic islet cells and endocrine pancreatic tumours
| Autor: | Asia Brodzky, Yael Herzog, Joel K. Greenson, Murray B. Resnick, Tzafra Cohen, Gera Neufeld, Samuel Eldar, Zoya Gluzman-Poltorak |
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| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_specialty
Pathology Pancreatic disease Glucagonoma Nerve Tissue Proteins Receptors Cell Surface Neuroendocrine tumors Biology Pathology and Forensic Medicine Diagnosis Differential Immunoenzyme Techniques Islets of Langerhans Internal medicine Biomarkers Tumor medicine Humans Pancreatic polypeptide Insulinoma geography geography.geographical_feature_category Pancreatic islets Adenoma Islet Cell medicine.disease Islet Neuropilin-1 Pancreatic Neoplasms Neuroendocrine Tumors Endocrinology medicine.anatomical_structure Gastrinoma Pancreas |
| Zdroj: | The Journal of Pathology. 198:77-82 |
| ISSN: | 1096-9896 0022-3417 |
| Popis: | Neuropilin-2 (NP-2) is a cell surface transmembrane protein originally characterized as a receptor for the type 3 semaphorins, and more recently for a number of vascular endothelial growth factor (VEGF) isoforms. NP-2 expression has been recently localized to a subset of neuroendocrine cells in the gastrointestinal tract. The aim of this study was to define the expression pattern of NP-2 in normal pancreatic islets and to determine the utility of NP-2 expression as a diagnostic marker of pancreatic endocrine tumours. Paraffin-embedded tissue sections from 30 endocrine pancreatic tumours (EPTs) and from normal pancreas were immunostained with a rabbit polyclonal antibody generated towards NP-2. Nineteen of the tumours were hormonally functional (nine insulinomas, nine gastrinomas, and one glucagonoma). The NP-2 staining pattern was correlated with islet cell hormone expression. In addition, NP-2 expression was evaluated in other normal neuroendocrine tissues and neuroendocrine neoplasms. In normal pancreas, NP-2 stained a distinct subset of islet cells situated primarily at the islet periphery. Double immunohistochemical staining revealed co-localization with glucagon-expressing cells. Moderate to strong NP-2 staining was present in 27 of 30 EPTs. Serial staining of the pancreatic tumours with insulin, gastrin, glucagon, pancreatic polypeptide (PP) or somatostatin did not reveal a distinct pattern of co-localization. NP-2 expression was not detected in neuroendocrine cells outside the gastroenteropancreatic system, or in their corresponding neoplasms, except for focal staining in one bronchial carcinoid tumour. In conclusion, the vast majority of EPTs examined expressed NP-2, suggesting its utility as a diagnostic marker for these tumours. The function of NP-2 in islet cell biology or tumourigenesis remains to be elucidated. |
| Databáze: | OpenAIRE |
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