Prevotella melaninogenica, a Sentinel Species of Antibiotic Resistance in Cystic Fibrosis Respiratory Niche?
| Autor: | Geneviève Héry-Arnaud, Elise Courteboeuf, S. Gouriou, Charles-Antoine Guilloux, Claudie Lamoureux, Clémence Beauruelle |
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| Přispěvatelé: | Génétique, génomique fonctionnelle et biotechnologies (UMR 1078) (GGB), EFS-Université de Brest (UBO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO), Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), PODEUR, Sophie |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Imipenem QH301-705.5 [SDV]Life Sciences [q-bio] 030106 microbiology Microbiology Tazobactam Article Prevotella melaninogenica cystic fibrosis resistance 03 medical and health sciences Antibiotic resistance antibiotics susceptibility Virology Clavulanic acid medicine Prevotella Biology (General) biology Clindamycin biology.organism_classification [SDV] Life Sciences [q-bio] 030104 developmental biology medicine.drug Piperacillin |
| Zdroj: | Microorganisms Volume 9 Issue 6 Microorganisms, MDPI, 2021, 9 (6), pp.1275. ⟨10.3390/microorganisms9061275⟩ Microorganisms, Vol 9, Iss 1275, p 1275 (2021) |
| ISSN: | 2076-2607 |
| DOI: | 10.3390/microorganisms9061275⟩ |
| Popis: | International audience; The importance and abundance of strict anaerobic bacteria in the respiratory microbiota of people with cystic fibrosis (PWCF) is now established through studies based on high-throughput sequencing or extended-culture methods. In CF respiratory niche, one of the most prevalent anaerobic genera is Prevotella, and particularly the species Prevotella melaninogenica. The objective of this study was to evaluate the antibiotic susceptibility of this anaerobic species. Fifty isolates of P. melaninogenica cultured from sputum of 50 PWCF have been included. Antibiotic susceptibility testing was performed using the agar diffusion method. All isolates were susceptible to the following antibiotics: amoxicillin/clavulanic acid, piperacillin/tazobactam, imipenem and metronidazole. A total of 96% of the isolates (48/50) were resistant to amoxicillin (indicating beta-lactamase production), 34% to clindamycin (17/50) and 24% to moxifloxacin (12/50). Moreover, 10% (5/50) were multidrug-resistant. A significant and positive correlation was found between clindamycin resistance and chronic azithromycin administration. This preliminary study on a predominant species of the lung “anaerobiome” shows high percentages of resistance, potentially exacerbated by the initiation of long-term antibiotic therapy in PWCF. The anaerobic resistome characterization, focusing on species rather than genera, is needed in the future to better prevent the emergence of resistance within lung microbiota. |
| Databáze: | OpenAIRE |
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