Transcriptional Changes in CD16+ Monocytes May Contribute to the Pathogenesis of COVID-19
| Autor: | Vanessa Chilunda, Pablo Martinez-Aguado, Li C. Xia, Laura Cheney, Aniella Murphy, Veronica Veksler, Vanessa Ruiz, Tina M. Calderon, Joan W. Berman |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male Transcription Genetic Severity of Illness Index Monocytes 0302 clinical medicine cell movement Interferon Immunology and Allergy RNA-Seq Original Research Middle Aged 030220 oncology & carcinogenesis Host-Pathogen Interactions Interferon Regulatory Factors Cytokines Female medicine.symptom Inflammation Mediators Single-Cell Analysis Cell activation medicine.drug Adult Antigen presentation Immunology Inflammation CD16 Biology GPI-Linked Proteins Peripheral blood mononuclear cell Mitochondrial Proteins 03 medical and health sciences Young Adult Immune system medicine Humans Aged Innate immune system SARS-CoV-2 Gene Expression Profiling Receptors IgG COVID-19 RC581-607 030104 developmental biology inflammation Case-Control Studies Immunologic diseases. Allergy non-classical monocytes Apoptosis Regulatory Proteins Transcriptome single-cell transcriptomics |
| Zdroj: | Frontiers in Immunology Frontiers in Immunology, Vol 12 (2021) |
| ISSN: | 1664-3224 |
| DOI: | 10.3389/fimmu.2021.665773 |
| Popis: | The COVID-19 pandemic has caused more than three million deaths globally. The severity of the disease is characterized, in part, by a dysregulated immune response. CD16+ monocytes are innate immune cells involved in inflammatory responses to viral infections, and tissue repair, among other functions. We characterized the transcriptional changes in CD16+ monocytes from PBMC of people with COVID-19, and from healthy individuals using publicly available single cell RNA sequencing data. CD16+ monocytes from people with COVID-19 compared to those from healthy individuals expressed transcriptional changes indicative of increased cell activation, and induction of a migratory phenotype. We also analyzed COVID-19 cases based on severity of the disease and found that mild cases were characterized by upregulation of interferon response and MHC class II related genes, whereas the severe cases had dysregulated expression of mitochondrial and antigen presentation genes, and upregulated inflammatory, cell movement, and apoptotic gene signatures. These results suggest that CD16+ monocytes in people with COVID-19 contribute to a dysregulated host response characterized by decreased antigen presentation, and an elevated inflammatory response with increased monocytic infiltration into tissues. Our results show that there are transcriptomic changes in CD16+ monocytes that may impact the functions of these cells, contributing to the pathogenesis and severity of COVID-19. |
| Databáze: | OpenAIRE |
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