A simple and efficient sample preparation for taxanes in Taxus chinensis needles with natural menthol-based aqueous deep eutectic solvent
| Autor: | Jiao Jiao, Xiao-Hong Fan, Ming-Zhu Dong, Mu-Jie Lv, Ya-Wei Zhu, Qing Yang, Yu-Jie Fu, Li-Tao Wang, Juan-Yan An, Dong Meng, Yuan-Hang Chang |
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| Rok vydání: | 2019 |
| Předmět: |
Filtration and Separation
01 natural sciences Analytical Chemistry Matrix (chemical analysis) chemistry.chemical_compound Sample preparation Chromatography High Pressure Liquid Aqueous solution Chromatography biology 010405 organic chemistry Chemistry 010401 analytical chemistry Extraction (chemistry) Water Repeatability biology.organism_classification Antineoplastic Agents Phytogenic 0104 chemical sciences Deep eutectic solvent Menthol Taxus Solvents Taxoids |
| Zdroj: | Journal of separation scienceREFERENCES. 43(7) |
| ISSN: | 1615-9314 |
| Popis: | Taxanes are natural anticancer constituents, and the sample preparation from matrix normally depends on organochlorine solvents. In this study, green and natural menthol-based aqueous deep eutectic solvent was synthesized and used for sample preparation for taxanes. Five key parameters were optimized and the optimal preparation conditions were as follows: menthol/1-propanol ratio 1:1 (mol/mol), solid-liquid ratio 1:30 g/mL, extraction time 30 min, ultrasonic power 250 W, and water content 80%. Under the above conditions, the total extraction efficiency of seven main taxanes was 1.25- to 1.44-fold to the conventional methods. In addition, a high-performance liquid chromatography method with C18 column was established for quantitation of seven main taxanes in 0.9986), precision (relative standard deviation < 3.00%), repeatability (relative standard deviation < 3.69%), and recovery (90.26-109.00%). This method performed the extraction, and enrichment processes simultaneously, and it had advantages such as high extraction efficiency, simple operation, low cost, and eco-friendliness. This work indicated that the natural menthol-based deep eutectic solvent aqueous could be an excellent alternative to the sample preparation from Taxus or other plants. |
| Databáze: | OpenAIRE |
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