Genetic vaccines protect against Sin Nombre hantavirus challenge in the deer mouse (Peromyscus maniculatus)
| Autor: | C. Richard Lyons, Katy Mirowsky, Chunyan Ye, Barbara Masten, Brian Hjelle, Mausumi Bharadwaj, Joyce Yee, Jason Botten |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
DNA
Complementary Sin Nombre virus Peromyscus Hantavirus Infections Antibodies Viral Injections Intramuscular Epitope Virus Mice Viral Envelope Proteins Antigen Virology Vaccines DNA medicine Animals Deer mouse medicine.vector_of_disease Nucleocapsid Neutralizing antibody biology Viral Vaccine Viral Vaccines biology.organism_classification biology.protein Spleen |
| Zdroj: | Journal of General Virology. 83:1745-1751 |
| ISSN: | 1465-2099 0022-1317 |
| Popis: | We used a deer mouse (Peromyscus maniculatus) infection model to test the protective efficacy of genetic vaccine candidates for Sin Nombre (SN) virus that were known to provoke immunological responses in BALB/c mice (Bharadwaj et al., Vaccine 17, 2836–2843, 1999). Protective epitopes were localized in each of four overlapping cDNA fragments that encoded portions of the SN virus G1 glycoprotein antigen; the nucleocapsid gene also was protective. The protective efficacy of glycoprotein gene fragments correlated with splenocyte proliferation in the presence of cognate antigen, but none induced neutralizing antibodies. Genetic vaccines against SN virus can protect outbred deer mice from infection even in the absence of a neutralizing antibody response. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|