Mechanisms of Bone Morphogenetic Protein-7 Protective Effects Against Cold Ischemia-Induced Renal Injury in Rats

Autor: Dragica Bobinac, Hrvoje Omrčen, Tanja Ćelić, Josip Španjol
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
heat-shock proteins
oxidative stress
mesenchymal transition
transplantation
storage
preservation
strategy
oxygen
cells
BMP-7
Adenosine
medicine.medical_treatment
Allopurinol
Bone Morphogenetic Protein 7
Organ Preservation Solutions
Delayed Graft Function
030204 cardiovascular system & hematology
Andrology
03 medical and health sciences
0302 clinical medicine
Raffinose
Renal injury
medicine
Animals
Insulin
Viaspan
Cold ischemia
Saline
Transplantation
Messenger RNA
Kidney
Chemistry
BIOMEDICINE AND HEALTHCARE. Basic Medical Sciences
Mesenchymal stem cell
Cold Ischemia
Glutathione
Kidney Transplantation
Rats
Bone morphogenetic protein 7
030104 developmental biology
medicine.anatomical_structure
Reperfusion Injury
Surgery
BIOMEDICINA I ZDRAVSTVO. Temeljne medicinske znanosti
Zdroj: Transplantation proceedings
Volume 50
Issue 10
ISSN: 1873-2623
0041-1345
Popis: Deceased donor kidneys are exposed to cold ischemic insult which makes them particularly susceptible to the effects of cold ischemic injury during hypothermic preservation resulting in high rates of delayed graft function. Bone morphogenetic protein-7 (BMP-7) is a valuable reagent in the field of tissue regeneration and preservation under ischemic conditions. Following these insights, we investigated the effect of recombinant human BMP-7 (rhBMP-7) on graft preservation during cold ischemia. The study was conducted on an experimental model of kidney cold ischemia in rats. Kidneys were perfused with University of Wisconsin (UW) saline solution, rhBMP-7, or rhBMP-7 + UW, and exposed to cold ischemia for 6, 12, and 24 hours. In tubular epithelial cells of kidneys perfused with rhBMP-7 and rhBMP-7+UW solution, the expression of BMP-7 and E-cadherin was observed after 24 hours of cold ischemia. In kidneys not perfused with rhBMP-7, high expression of transforming growth factor-β and α-smooth muscle actin was found. Also, in kidneys perfused with rhBMP-7 solution, statistically higher levels of Smad1, Smad5, and Smad8 messenger RNA expressions were proven. BMP-7 maintains the morphology of kidney tissue better than UW solution during 24 hours of cold ischemia. BMP-7 prevents epithelial to mesenchymal transformation and consequently maintains epithelial phenotype of tubular cells.
Databáze: OpenAIRE
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