The effects of N-acetylcysteine on radiotherapy-induced small intestinal damage in rats
| Autor: | Levent Tumkaya, Adnan Yilmaz, Atilla Topcu, Sema Yilmaz Rakici, Filiz Mercantepe |
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| Rok vydání: | 2019 |
| Předmět: |
Male
medicine.medical_treatment Caspase 3 medicine.disease_cause General Biochemistry Genetics and Molecular Biology Ionizing radiation Rats Sprague-Dawley Acetylcysteine Intestine Small medicine Animals Radiation Injuries Original Research Radiotherapy business.industry Cancer Free Radical Scavengers medicine.disease Six million Rats Radiation therapy Oxidative Stress Apoptosis Cancer research business Oxidative stress medicine.drug |
| Zdroj: | Experimental Biology and Medicine. 244:372-379 |
| ISSN: | 1535-3699 1535-3702 |
| Popis: | Six million cancer patients worldwide receive radiotherapy, either alone or in combination with other cancer treatments annually. The purpose of this study was to investigate, at the structural and molecular levels, the protective effect of N-acetylcysteine (NAC), a powerful antioxidant, against radiotherapy-induced damage on the intestinal system. Three study groups were constituted: control (group 1: no radiotherapy), radiotherapy (RT; group 2), and RT + NAC (group 3). The NAC group received 300 mg/kg NAC orally for five days before irradiation. At the end of the 5th day, 6 Gy was irradiated once, and 300 mg/kg NAC was administered orally for two days following irradiation. NAC drug administration was maintained for a total of seven days. At the end of the study, the rats were euthanized by the administration of anesthetic agents and sacrificed. The malondialdehyde (MDA) levels and caspase-3 expression increased and glutathione (GSH) levels decreased in RT rats compared to the control group ( p Impact statement Some six million cancer patients currently receive radiotherapy. Radiotherapy eliminates cancer cells by accelerating their death. However, radiotherapy is not selective, and it therefore harms healthy tissues around cancerous tissue. The latest studies have shown that the irradiation of biological materials causes a rapid increase in reactive oxygen species (ROS) in the tissue as a result of exposure of the target molecule to direct and indirect ionization. N-acetylcysteine (NAC) is an antioxidant that permits the elimination of free oxygen radicals and that contributes to glutathione synthesis. Our study, therefore, examined the effects of radiation resulting from radiotherapy on the small intestine at the molecular level, and prospectively considered the potential protective characteristics of NAC against gastrointestinal syndrome resulting from radiotherapy. |
| Databáze: | OpenAIRE |
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