Vascular perfusion kinetics by contrast-enhanced ultrasound are related to synovial microvascularity in the joints of psoriatic arthritis
| Autor: | David Sacerdoti, Valeria Beltrame, Francesca Lunardi, Ugo Fiocco, Leonardo Punzi, Roberto Stramare, Fulvia Chieco Bianchi, Fiorella Calabrese, Francesco Caso, Leopoldo Rubaltelli, Elena Scagliori, Francesca Oliviero, Raffaele Scarpa, Veronica Martini, Luisa Costa, Alessandro Coran, Andrea Doria, Enrico Grisan, L Cozzi, Anna Scanu, M. Vezzù, Daniele Boso |
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| Přispěvatelé: | Fiocco, Ugo, Stramare, Roberto, Coran, Alessandro, Grisan, Enrico, Scagliori, Elena, Caso, Francesco, Costa, Luisa, Lunardi, Francesca, Oliviero, Francesca, Bianchi, Fulvia Chieco, Scanu, Anna, Martini, Veronica, Boso, Daniele, Beltrame, Valeria, Vezzù, Maristella, Cozzi, Luisella, Scarpa, Raffaele, Sacerdoti, David, Punzi, Leonardo, Doria, Andrea, Calabrese, Fiorella, Rubaltelli, Leopoldo |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Knee Joint Biopsy Sulfur Hexafluoride Synovial hypervascularity Contrast Media Refilling time (RT) Arthroscopy Vascularity Rheumatology Psoriatic arthritis (PsA) medicine Humans synovial hystology psoriatic arthrites CEUS Kinetic business.industry Medicine (all) Ultrasound Vascular perfusion kinetic Arthritis Psoriatic Synovial Membrane Ultrasonography Doppler General Medicine Blood flow Hypervascularity Middle Aged Kinetics Immunohistochemical (IHC) quantitative estimation Synovial Cell psoriatic arthrites CEUS synovial hystology Regional Blood Flow Microbubbles Contrast-enhanced harmonic ultrasound (CEUS) Female medicine.symptom business Perfusion Contrast-enhanced ultrasound Human |
| Popis: | The purpose of the study was to assess the relationship of the continuous mode contrast-enhanced harmonic ultrasound (CEUS) imaging with the histopathological and immunohistochemical (IHC) quantitative estimation of microvascular proliferation on synovial samples of patients affected by sustained psoriatic arthritis (PsA). A dedicated linear transducer was used in conjunction with a specific continuous mode contrast enhanced harmonic imaging technology with a second-generation sulfur hexafluoride-filled microbubbles C-agent. The examination was carried out within 1 week before arthroscopic biopsies in 32 active joints. Perfusional parameters were analyzed including regional blood flow (RBF); peak (PEAK) of the C-signal intensity, proportional to the regional blood volume (RBV); beta (β) perfusion frequency; slope (S), representing the inclination of the tangent in the origin; and the refilling time (RT), the reverse of beta. Arthroscopic synovial biopsies were targeted in the hypervascularity areas, as in the same knee recesses assessed by CEUS; the synovial cell infiltrate and vascularity (vessel density) was evaluated by IHC staining of CD45 (mononuclear cell) and CD31, CD105 (endothelial cell) markers, measured by computer-assisted morphometric analysis. In the CEUS area examined, the corresponding time-intensity curves demonstrated a slow rise time. Synovial histology showed slight increased layer lining thickness, perivascular lymphomonocyte cell infiltration, and microvascular remodeling, with marked vessel wall thickening with reduction of the vascular lumen. A significant correlation was found between RT and CD31+ as PEAK and CD105+ vessel density; RT was inversely correlated to RBF, PEAK, S, and β. The study demonstrated the association of the CEUS perfusion kinetics with the histopathological quantitative and morphologic estimation of synovial microvascular proliferation, suggesting that a CEUS imaging represents a reliable tool for the estimate of the synovial hypervascularity in PsA. |
| Databáze: | OpenAIRE |
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