Patterns of cellular injury in myocardial ischemia determined by monoclonal antimyosin
| Autor: | William A. Clark, R Zak, A C Nolan, T Karwoski |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Pathology
medicine.medical_specialty Ischemia Fluorescent Antibody Technique Coronary Disease Enzyme-Linked Immunosorbent Assay Myosins Immunofluorescence Mice Occlusion Myosin medicine Animals Mice Inbred BALB C Multidisciplinary medicine.diagnostic_test business.industry Myocardium Cardiac muscle Antibodies Monoclonal Rats Inbred Strains Anatomy medicine.disease Rats Staining medicine.anatomical_structure Ventricle Monoclonal business Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 80:6046-6050 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | The development of cellular injury in the rat left ventricle resulting from left coronary artery occlusion was examined by immunofluorescence after intravenous injection of monoclonal antimyosin. Cardiac muscle cells that bound antimyosin during ischemia were localized by staining sections with fluorescein-conjugated anti-mouse IgG. Fluorescent staining was detectable within the ischemic region of the left ventricle 3 hr after occlusion and injection of antimyosin. After 6 hr of ischemia, the highly irregular margin of the ischemic zone was clearly outlined by fluorescent cells. At 3-6 hr after occlusion, marked heterogeneity in cellular staining was observed in the epicardial half of the ischemic area, with intensely fluorescent cells intermixed with cells of markedly lower fluorescence. By 24 hr, a homogeneous pattern of staining was observed throughout the ischemic zone. In nonischemic regions of the heart and in rats treated for 24 hr with antimyosin without occlusion, there were only background levels of staining. We conclude that: (i) visualization of ischemic cells via antimyosin provides a sensitive means for examining developing patterns of injury; (ii) the heterogeneity of staining during early ischemia may reflect variation in cellular resistance to deprivation; and (iii) the pattern of fluorescence at the margin of the occluded region indicates that the "border zone" is composed of interdigitating ischemic and nonischemic tissues. |
| Databáze: | OpenAIRE |
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