The cDNA sequence and expression of an ubiquitin-tail gene fusion in Neurospora crassa
| Autor: | Erich Grotewold, G. E. Taccioli, G. O. Aisemberg, Norberte D. Judewicz |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Ribosomal Proteins
Genes Fungal Molecular Sequence Data Restriction Mapping Neurospora crassa Fusion gene Ribosomal protein Gene Expression Regulation Fungal Sequence Homology Nucleic Acid Complementary DNA Gene expression Genetics Amino Acid Sequence Cloning Molecular Cycloheximide Ubiquitins Base Sequence biology fungi Nucleic acid sequence Crassa General Medicine Blotting Northern biology.organism_classification Molecular biology Cell biology Blotting Southern Fusion transcript |
| Zdroj: | Gene. 102:133-137 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/0378-1119(91)90552-m |
| Popis: | The genome of Neurospora crassa contains at least one natural fusion gene encoding a single ubiquitin (UBI) unit with a 78-amino acid C-terminal extension. The predominantly basic tail sequence corresponds to a highly conserved ribosomal protein identified in other organisms. The 0.7-kb UBI fusion transcript is mainly expressed in germinating conidia and other stages of active cell replication. Under starvation conditions attained by nutrient depletion, or after polyamine depletion, the UBI fusion gene is shut off while the polyUBI transcript is preserved. Cycloheximide addition promotes polyUBI, but not UBI fusion transcript accumulation in N. crassa. |
| Databáze: | OpenAIRE |
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