Capsaicin Exerts Anti-convulsant and Neuroprotective Effects in Pentylenetetrazole-Induced Seizures
| Autor: | Amany A. Sleem, Nermeen M. Shaffie, Omar M.E. Abdel-Salam, Marawan Abd El Baset Mohamed Sayed, Eman R. Youness |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Agonist Phenytoin medicine.drug_class TRPV1 Status epilepticus Pharmacology Biochemistry Neuroprotection Nitric oxide Rats Sprague-Dawley Random Allocation 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound 0302 clinical medicine Seizures otorhinolaryngologic diseases Animals Medicine heterocyclic compounds Pentylenetetrazol Dose-Response Relationship Drug business.industry digestive oral and skin physiology Brain General Medicine Rats nervous system diseases Neuroprotective Agents 030104 developmental biology chemistry Capsaicin Pentylenetetrazole Anticonvulsants lipids (amino acids peptides and proteins) Lipid Peroxidation medicine.symptom business 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Neurochemical Research. 45:1045-1061 |
| ISSN: | 1573-6903 0364-3190 |
| DOI: | 10.1007/s11064-020-02979-3 |
| Popis: | The transient receptor potential vanilloid-1 (TRPV1) receptor has been implicated in the development of epileptic seizures. We examined the effect of the TRPV1 agonist capsaicin on epileptic seizures, neuronal injury and oxidative stress in a model of status epilepticus induced in the rat by intraperitoneal (i.p.) injections of pentylenetetrazole (PTZ). Capsaicin was i.p. given at 1 or 2 mg/kg, 30 min before the first PTZ injection. Other groups were i.p. treated with the vehicle or the anti-epileptic drug phenytoin (30 mg/kg) alone or co-administered with capsaicin at 2 mg/kg. Brain levels of malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide, and paraoxonase-1 (PON-1) activity, seizure scores, latency time and PTZ dose required to reach status epilepticus were determined. Histopathological assessment of neuronal damage was done. Results showed that brain MDA decreased by treatment with capsaicin, phenytoin or capsaicin/phenytoin. Nitric oxide decreased by capsaicin or capsaicin/phenytoin. GSH and PON-1 activity increased after capsaicin, phenytoin or capsaicin/phenytoin. Mean total seizure score decreased by 48.8% and 66.3% by capsaicin compared with 78.7% for phenytoin and 69.8% for capsaicin/phenytoin treatment. Only phenytoin increased the latency (115.7%) and threshold dose of PTZ (78.3%). Capsaicin did not decrease the anti-convulsive effect of phenytoin but prevented the phenytoin-induced increase in latency time and threshold dose. Neuronal damage decreased by phenytoin or capsaicin at 2 mg/kg but almost completely prevented by capsaicin/phenytoin. Thus in this model of status epilepticus, capsaicin decreased brain oxidative stress, the severity of seizures and neuronal injury and its co-administration with phenytoin afforded neuronal protection. |
| Databáze: | OpenAIRE |
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