Structural Coupling Throughout the Active Site Hydrogen Bond Networks of Ketosteroid Isomerase and Photoactive Yellow Protein
| Autor: | Filip Yabukarski, Todd J. Martínez, Ruibin Liang, Tzanko Doukov, Margaux M. Pinney, Daniel Herschlag, Aditya Natarajan, Fang Liu, David M. Sanchez, Jason P. Schwans |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Stereochemistry Proton Magnetic Resonance Spectroscopy Steroid Isomerases Isomerase Crystallography X-Ray Photoreceptors Microbial 010402 general chemistry 01 natural sciences Biochemistry Catalysis 03 medical and health sciences chemistry.chemical_compound Colloid and Surface Chemistry Molecular recognition Bacterial Proteins Catalytic Domain Ketosteroid biology Chemistry Hydrogen bond Active site Hydrogen Bonding General Chemistry Ketosteroids 0104 chemical sciences Folding (chemistry) 030104 developmental biology Proton NMR biology.protein Oxyanion hole |
| Zdroj: | Journal of the American Chemical Society. 140:9827-9843 |
| ISSN: | 1520-5126 0002-7863 |
| Popis: | Hydrogen bonds are fundamental to biological systems and are regularly found in networks implicated in folding, molecular recognition, catalysis, and allostery. Given their ubiquity, we asked the fundamental questions of whether, and to what extent, hydrogen bonds within networks are structurally coupled. To address these questions, we turned to three protein systems, two variants of ketosteroid isomerase and one of photoactive yellow protein. We perturbed their hydrogen bond networks via a combination of site-directed mutagenesis and unnatural amino acid substitution, and we used 1H NMR and high-resolution X-ray crystallography to determine the effects of these perturbations on the lengths of the two oxyanion hole hydrogen bonds that are donated to negatively charged transition state analogs. Perturbations that lengthened or shortened one of the oxyanion hole hydrogen bonds had the opposite effect on the other. The oxyanion hole hydrogen bonds were also affected by distal hydrogen bonds in the network, w... |
| Databáze: | OpenAIRE |
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