Expression of topoisomerase III α in normal and neoplastic tissues determined by immunohistochemistry using novel monoclonal antibody
| Autor: | M Steward, John Anderson, C. H. W. Horne, A J Lodge, B. Haugk, F Fenwick, B. Angus, S W Ng |
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| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Gene isoform
Cancer Research medicine.drug_class Blotting Western Monoclonal antibody law.invention chemistry.chemical_compound Mice Transcription (biology) law Neoplasms expression medicine Animals Humans tissues Tissue Distribution chemistry.chemical_classification Paraffin Embedding biology Topoisomerase Antibodies Monoclonal Regular Article Molecular biology Immunohistochemistry Recombinant Proteins Enzyme Oncology chemistry DNA Topoisomerases Type I monoclonal antibody topoisomerase IIIα biology.protein Recombinant DNA Female HT29 Cells DNA HeLa Cells |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| Popis: | Topoisomerases are nuclear enzymes that modulate the topological structure of DNA in order to facilitate cellular events such as replication and transcription. These enzymes are also the cellular targets of certain classes of chemotherapeutic agents termed topoisomerase poisons. A new human topoisomerase isoform, IIIa, was discovered in 1996, which is thought to have roles in genome stability and possibly chromosome separation during mitosis. It is possible that novel or existing anti-topoisomerase agents target topoisomerase IIIa, suggesting that this enzyme may have potential as a prognostic marker and chemotherapeutic target. In order to study expression patterns of topoisomerase IIIa we have produced a novel monoclonal antibody to human topoisomerase IIIa (TOPO3a-1A4), and used it to assess topoisomerase IIIa expression in a wide range of normal and neoplastic tissues. We have found that topoisomerase IIIa is expressed in a wide range of tissue types, with especially high concentrations in endothelial cells and stromal fibroblasts. No general relationship was observed between expression of topoisomerase IIIa and proliferation. Expression in neoplastic tissues often paralleled their normal counterparts, although certain tumours showed either increased (e.g. colonic adenoma) or reduced (e.g. gastric carcinoma, small cell carcinoma of bronchus) expression. If topoisomerase IIIa is found to be a target for chemotherapeutic agents, clinical response in different tumour types may be related to topoisomerase IIIa expression, which may be assessed using TOPO3a-1A4. © 2000 Cancer Research Campaign |
| Databáze: | OpenAIRE |
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