Additional file 1: of Real-time tracking of complex ubiquitination cascades using a fluorescent confocal on-bead assay

Autor: Koszela, Joanna, Pham, Nhan, Evans, David, Mann, Stefan, Perez-Pi, Irene, Shave, Steven, Ceccarelli, Derek, Sicheri, Frank, Tyers, Mike, Auer, Manfred
Rok vydání: 2018
DOI: 10.6084/m9.figshare.6957845
Popis: Contains Supplementary Figures S1-S7. Figure S1. Ring fluorescence intensity is linearly proportional to the amount of bead-bound fluorescent substrate and is consistent across a test plate. Figure S2. On-bead ubiquitination of a protein substrate, p53. Figure S3. Fluorescence excitation and emission spectra for simultaneous detection of three different fluorescent-fusion proteins. Figure S4. Bead detection and analysis allows for differential detection of mixed small and large bead populations. Figure S5. Inhibitory effects observed in time-resolved ubiquitination cascade reactions. Figure S6. Concentration-dependent inhibitory activity of BAY 11-7082 observed on eGFP-Ube2L3 using UPS-CONA. Figure S7. Ring fluorescence intensity is stable over time and remains linearly proportional to the amount of bead-bound fluorescent substrate. Table S1. UPS-CONA is a broadly applicable assay. (DOCX 6279 kb)
Databáze: OpenAIRE