Effect of Azadinium spinosum on the feeding behaviour and azaspiracid accumulation of Mytilus edulis
| Autor: | Véronique Séchet, Christine Herrenknecht, Urban Tillmann, Andrea Contreras, Philipp Hess, Philippe Truquet, Thierry Jauffrais |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
0106 biological sciences
animal structures Metabolic Clearance Rate Mytilus edulis Health Toxicology and Mutagenesis Ecophysiology ved/biology.organism_classification_rank.species Aquatic Science Azadinium spinosum 01 natural sciences Poisons 03 medical and health sciences AZA accumulation Botany Animals Azaspiracid Spiro Compounds Food science Stratum spinosum Biotransformation 030304 developmental biology 0303 health sciences biology Dinoflagellate ved/biology 010604 marine biology & hydrobiology fungi Feeding Behavior Mussel biology.organism_classification Bivalve molluscs Mytilus AZA biotransformation Dinoflagellida Marine Toxins Trophic transfer Clearance rate Blue mussel |
| Zdroj: | Aquatic Toxicology (0166-445X) (Elsevier Science Bv), 2012-11, Vol. 124, P. 179-187 |
| Popis: | Azadinium spinosum , a small toxic dinoflagellate, was recently isolated and identified as a primary producer of azaspiracid toxins (AZAs). Previous experiments related to AZA accumulation in blue mussels upon direct feeding with A. spinosum revealed increased mussel mortality and had negative effects on the thickness of the digestive gland tubules. Therefore we conducted follow up experiments in order to study effects of A. spinosum on mussel feeding behaviour. Individual assessment of mussel feeding time activity (FTA), clearance rate (CR), filtration rate (TFR), absorption rate (AR), faeces and pseudofaeces production were carried out on mussel fed either toxic ( A. spinosum ) or non-toxic ( Isochrisis aff. galbana (T- Iso )) diets. Furthermore, AZA accumulation and biotransformation in mussels were followed using liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS). A. spinosum had a significant effect on mussel feeding behaviour compared to T- Iso : CR was lower by a factor of 6, FTA by a factor of 5, TFR by a factor of 3 and AR even decreased to negative values for the last day of exposure. Even so, a rapid AZA accumulation was observed during the first hours of the trial; less than 6 h of feeding were required to reach AZA concentration in mussel above regulatory level. In consistence with physiological observations, AZA concentration of about 200 μg kg −1 did not increase further until the end of the study. AZA bioconversion was also found to be a fast process: after 3 h of exposure AZA17, -19 and AZA7-10 were already found, with a proportion of AZA17 equal to AZA2. These results show a negative effect of A. spinosum on blue mussel feeding activity and indicate a possible regulation of AZA uptake by decreasing filtration and increasing pseudofaeces production. |
| Databáze: | OpenAIRE |
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