Time–dose relationships in radiation-enhanced integration
| Autor: | C. W. Stevens, G. J. Cerniglia, M. Puppi |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Time Factors
Radiobiology Cell Survival medicine.medical_treatment Fractionation Biology Radiation Transfection Cell Line Ionizing radiation Mice medicine Animals Humans Radiology Nuclear Medicine and imaging Irradiation Cytotoxicity Recombination Genetic Radiological and Ultrasound Technology business.industry Dose-Response Relationship Radiation 3T3 Cells Radiation therapy Biophysics Nuclear medicine business DNA Damage Plasmids |
| Zdroj: | International Journal of Radiation Biology. 77:841-846 |
| ISSN: | 1362-3095 0955-3002 |
| DOI: | 10.1080/09553000110053882 |
| Popis: | We have shown that ionizing radiation increases recombination, as manifested by increased stable transduction of both plasmid and adenoviral vectors. This paper reports the duration of increased recombination after irradiation.A549 or NIH/3T3 cells were transfected at various times after irradiation. Cells were also irradiated with several fractionation schemes and then transfected.Enhanced integration (EI) is a very long-lived process, lasting at least 2-3 days after single radiation fractions. The duration of EI activation is radiation dose-dependent. The efficiency of EI is dependent on radiation dose and independent of fractionation, such that low dose-rate, fractionated and single radiation doses result in similar levels of EI when corrected for differences in cytotoxicity.Radiation, given with fraction sizes and dose-rates used in clinical radiation therapy, induces a long-lived hyper-recombination state. Since radiotherapy is already a component of treatment for many malignancies and is integrated into radiation-gene therapy trials, an understanding of recombination events that improve gene delivery is important and timely. |
| Databáze: | OpenAIRE |
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