Mobility of the hepatitis C virus NS4B protein on the endoplasmic reticulum membrane and membrane-associated foci
| Autor: | John McLauchlan, Annette I. Taylor, Sarah Gretton |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Recombinant Fusion Proteins
viruses Hepatitis C virus Green Fluorescent Proteins Hepacivirus Viral Nonstructural Proteins Biology Endoplasmic Reticulum Virus Replication medicine.disease_cause Virus Genes Reporter Cricetinae Virology medicine Animals Humans Cells Cultured Microscopy Confocal Endoplasmic reticulum Vesicle Cytoplasmic Vesicles RNA Molecular biology Fusion protein Membrane protein Viral replication RNA Viral |
| Zdroj: | Journal of General Virology. 86:1415-1421 |
| ISSN: | 1465-2099 0022-1317 |
| Popis: | The hepatitis C virus (HCV) non-structural protein NS4B induces morphological changes in the endoplasmic reticulum (ER) membrane that may have a direct role in viral RNA replication. A chimeric GFP–NS4B fusion protein located to the ER membrane and to foci that were attached to the ER. Thesemembrane-associatedfoci (MAFs) could be related to the membrane alterations observed in cells that replicate HCV RNA. The relationship of MAFs to pre-existing cellular structures is not known. Indirect immunofluorescence analysis demonstrated that they did not contain a cellular marker for vesicles, which have been implicated in the replication of other viruses. From photobleaching studies to examine diffusion of NS4B, the GFP-tagged protein had reduced mobility on MAFs compared with on the ER membrane. This slower mobility suggested that NS4B is likely to form different interactions on MAFs and the ER. |
| Databáze: | OpenAIRE |
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