The Rpd3-Sin3 Histone Deacetylase Regulates Replication Timing and Enables Intra-S Origin Control in Saccharomyces cerevisiae
| Autor: | Christopher J. Viggiani, Jennifer G. Aparicio, Daniel G. Gibson, Oscar M. Aparicio |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
DNA Replication
Saccharomyces cerevisiae Proteins Cell Cycle Proteins Saccharomyces cerevisiae Protein Serine-Threonine Kinases Histone Deacetylases S Phase Transcription (biology) Gene Expression Regulation Fungal Molecular Biology Gene Regulation of gene expression Genetics Replication timing biology Intracellular Signaling Peptides and Proteins Cell Biology DNA Dynamics and Chromosome Structure Chromatin Repressor Proteins Checkpoint Kinase 2 Sin3 Histone Deacetylase and Corepressor Complex Histone biology.protein Origin recognition complex Histone deacetylase Transcription Initiation Site Transcription Factors |
| Zdroj: | Molecular and Cellular Biology. 24:4769-4780 |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.24.11.4769-4780.2004 |
| Popis: | The replication of eukaryotic genomes follows a temporally staged program, in which late origin firing often occurs within domains of altered chromatin structure(s) and silenced genes. Histone deacetylation functions in gene silencing in some late-replicating regions, prompting an investigation of the role of histone deacetylation in replication timing control in Saccharomyces cerevisiae. Deletion of the histone deacetylase Rpd3 or its interacting partner Sin3 caused early activation of late origins at internal chromosomal loci but did not alter the initiation timing of early origins or a late-firing, telomere-proximal origin. By delaying initiation relative to the earliest origins, Rpd3 enables regulation of late origins by the intra-S replication checkpoint. RPD3 deletion suppresses the slow S phase of clb5Delta cells by enabling late origins to fire earlier, suggesting that Rpd3 modulates the initiation timing of many origins throughout the genome. Examination of factors such as Ume6 that function together with Rpd3 in transcriptional repression indicates that Rpd3 regulates origin initiation timing independently of its role in transcriptional repression. This supports growing evidence that for much of the S. cerevisiae genome transcription and replication timing are not linked. |
| Databáze: | OpenAIRE |
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