Neutrophils Establish Rapid and Robust WAVE Complex Polarity in an Actin-Dependent Fashion
| Autor: | Orion D. Weiner, Sheel N. Dandekar, Andrew R. Houk, Arthur Millius |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Polarity (physics)
Neutrophils Blotting Western macromolecular substances Biology Cell Fractionation General Biochemistry Genetics and Molecular Biology Article Quantitative Biology::Cell Behavior Cell Line Quantitative Biology::Subcellular Processes 03 medical and health sciences Mice Micromanipulation 0302 clinical medicine Cell polarity Animals Actin 030304 developmental biology 0303 health sciences Microscopy Video Agricultural and Biological Sciences(all) Biochemistry Genetics and Molecular Biology(all) Dynamics (mechanics) Cell Polarity Chemotaxis Polarization (waves) Bridged Bicyclo Compounds Heterocyclic Actins Cell biology Wiskott-Aldrich Syndrome Protein Family Multiprotein Complexes Thiazolidines CELLBIO General Agricultural and Biological Sciences WAVE complex 030217 neurology & neurosurgery Intracellular |
| Zdroj: | Current Biology. (3):253-259 |
| ISSN: | 0960-9822 |
| DOI: | 10.1016/j.cub.2008.12.044 |
| Popis: | Asymmetric intracellular signals enable cells to migrate in response to external cues. The multiprotein WAVE (SCAR/WASF) complex activates the actin-nucleating Arp2/3 complex [1-4] and localizes to propagating “waves”, which direct actin assembly during neutrophil migration [5, 6]. Here, we observe similar WAVE complex dynamics in other mammalian cells and analyze WAVE complex dynamics during the establishment of neutrophil polarity. Earlier models proposed that either spatially-biased generation [7] or selection of protrusions [8] enables chemotaxis. These models require existing morphological polarity to control protrusions. Similar spatially-biased generation and selection of WAVE complex recruitment occur in morphologically unpolarized neutrophils during the development of their first protrusions. Additionally, several mechanisms limit WAVE complex recruitment during polarization and movement: intrinsic cues restrict WAVE complex distribution during the establishment of polarity, and asymmetric intracellular signals constrain WAVE complex distribution in morphologically polarized cells. External gradients can overcome both intrinsic biases and control WAVE complex localization. Following latrunculin-mediated inhibition of actin polymerization, addition and removal of agonist gradients globally recruits and releases the WAVE complex from the membrane. Under these conditions the WAVE complex no longer polarizes, despite the presence of strong external gradients. Thus, actin polymer and the WAVE complex reciprocally interact during polarization. |
| Databáze: | OpenAIRE |
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