Induction of cytotoxic T-lymphocytes specific for bovine herpesvirus-1 by DNA immunization

Autor: Muralidhar S. Deshpande, Nagendra R. Hegde, Mangala J Hariharan, Thanuja C Ambagala, S. Srikumaran, Manjula Navaratnam
Rok vydání: 2002
Předmět:
Cell Extracts
Cytotoxicity
Immunologic
Sindbis virus
Cellular immunity
Immunogen
Injections
Intradermal
animal diseases
viruses
Genetic Vectors
chemical and pharmacologic phenomena
Lymphocyte Activation
medicine.disease_cause
Major histocompatibility complex
Injections
Intramuscular
Herpesviridae
Virus
Cell Line
Mice
Viral Proteins
L Cells
Transduction
Genetic
Tumor Cells
Cultured
Vaccines
DNA
medicine
Animals
Cytotoxic T cell
Herpesvirus 1
Bovine
Mice
Inbred BALB C
Vaccines
Synthetic
General Veterinary
General Immunology and Microbiology
biology
Public Health
Environmental and Occupational Health
virus diseases
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
Virology
Bovine herpesvirus 1
Infectious Diseases
Culture Media
Conditioned
DNA
Viral
biology.protein
Molecular Medicine
Cattle
Female
Sindbis Virus
T-Lymphocytes
Cytotoxic
Zdroj: Vaccine. 20:3744-3751
ISSN: 0264-410X
DOI: 10.1016/s0264-410x(02)00375-4
Popis: Cytotoxic T-lymphocytes (CTLs) are critical for the defense against herpesvirus infections, in which cell-to-cell spread occurs earlier than the hematogenous spread. The ability of bovine herpesvirus-1 (BHV-1) to undergo latency, to induce apoptosis of CD4(+) T-lymphocytes, and to down-regulate the expression of major histocompatibility complex (MHC) class I molecules, necessitates the development of immunization strategies that do not involve the live virus. The objective of this study was to evaluate the feasibility of DNA immunization as a means of induction of CTLs against BHV-1. Mice were injected either by intramuscular (IM) or intradermal (ID) route with a Sindbis virus-based plasmid carrying the gene encoding the glycoprotein D (gD) of BHV-1. Splenocytes from the immunized mice were re-stimulated in vitro with gD-transduced syngeneic fibroblasts. The CTLs generated specifically lysed syngeneic targets, either transduced with gD or infected with BHV-1. IM route of inoculation induced a better CTL response when compared to ID route with respect to onset, magnitude and duration of immunity. These results indicate the feasibility of using a plasmid carrying the gene encoding BHV-1 gD as an immunogen to induce CTLs against BHV-1.
Databáze: OpenAIRE
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