MicroRNA-10b targets E-cadherin and modulates breast cancer metastasis
| Autor: | San-Yuan Sun, Jing Zhao, Qing-Song Xi, Yu Zhang, Shiying Yu, Pei-Ying Zhang, Yong Liu |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Molecular Sequence Data Down-Regulation Breast Neoplasms Biology Metastasis Breast cancer breast cancer Downregulation and upregulation Antigens CD Cell Line Tumor microRNA medicine Humans metastasis Neoplasm Invasiveness Neoplasm Metastasis skin and connective tissue diseases Aged Neoplasm Staging Regulation of gene expression Base Sequence Mechanism (biology) Cadherin microRNA-10b E-cadherin Breast cancer metastasis General Medicine Middle Aged medicine.disease Cadherins Up-Regulation Gene Expression Regulation Neoplastic MicroRNAs Basic Research Immunology Multivariate Analysis Cancer research Linear Models Female |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 |
| Popis: | Summary Background Recent studies have suggested that microRNA-10b (miR-10b) acts as a promoter of metastasis in breast cancer, although the underlying mechanism remains largely unknown. In this study, we provide the first evidence that E-cadherin (E-cad) is a potential target of miR-10b. Material/Method By applying gain-of-function and loss-of-function approaches in the metastatic breast cancer cell line MDA-MB-231, we demonstrated that miR-10b is necessary and sufficient to regulate the cellular expression of E-cad and in vitro tumor cell invasion. Results Comparative expression analysis of miR-10b in benign breast lesions (N=16), primary breast cancers (N=21), and metastatic breast carcinomas (N=23) revealed that miR-10b transcription was uniquely up-regulated in metastatic cancers. The expression level of miR-10b positively correlated with tumor size, pathological grading, clinical staging, lymph node metastasis, Her2-positivity and tumor proliferation, but was negatively associated with estrogen receptor-positivity, progesterone receptor-positivity and E-cad mRNA and protein levels. Conclusions These findings indicate the existence of a novel E-cadherin-related mechanism by which miR-10b modulates breast cancer metastasis. In addition, miR-10b may be a useful biomarker of advanced progression and metastasis of breast cancer. |
| Databáze: | OpenAIRE |
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