Identification of a potentially functional circRNA-miRNA-mRNA ceRNA regulatory network in bladder cancer by analysis of microarray data
| Autor: | Yue-Wei Yin, Chang-Bao Qu, Wen-yong Xue, Bao-Sai Lu, Jin-Chun Qi, Xin Wang, Yan-Ping Zhang, Jiang-hua Jia, Lei Du |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Messenger RNA CENPA Microarray Competing endogenous RNA Microarray analysis techniques Urology Computational biology Biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Reproductive Medicine 030220 oncology & carcinogenesis microRNA Original Article Gene HIST1H3H |
| Zdroj: | Transl Androl Urol |
| ISSN: | 2223-4691 2223-4683 |
| DOI: | 10.21037/tau-20-660 |
| Popis: | Background Circular RNAs (circRNAs) have received increasing attention in cancer development. However, a substantial number of circRNAs still require characterization. The purpose of this study is to uncover novel circRNAs and their molecular mechanism in bladder cancer (BCa). Methods A combinative strategy of extensive data mining and computational biology was employed to identify BCa-related circRNAs and explore their potential mechanisms of action. Results Three differentially expressed circRNAs (has_circ_0023642, has_circ_0047322, has_circ_0041151) were obtained from the microarray dataset (GSE92675). Four miRNAs (miR-616, miR-515-5p, miR-647, miR-1178) with potential binding sites with these three circRNAs were identified. Pathway analysis demonstrated that all four miRNAs were closely associated with some cancer-related pathways. Survival analysis indicated that these miRNAs might potentially play a role in tumor-suppressive functions in BCa. Subsequently, 181 overlapping genes were identified from 472 up-regulated genes in BCa (TCGA database), and 10,017 predicted target genes of the four miRNAs obtained. A circRNA-miRNA-mRNA network was constructed on the identified three circRNAs, four miRNAs, and 181 overlapping genes. Besides, six hub genes (CENPA, HIST1H2BJ, HIST1H2BO, HIST1H3H, HIST1H3B, HIST1H3F) were identified from establishing a protein-protein interaction (PPI) network on the same overlapping genes. Furthermore, a circRNA-miRNA-hub gene sub-network was built to delineate the links among the differential circRNAs, miRNA, and hub genes. Conclusions Our study provided significant insights into the molecular mechanisms that regulate the progression of BCa from the circRNA-miRNA-mRNA network view. |
| Databáze: | OpenAIRE |
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