Glucose responsive insulin production from human embryonic germ (EG) cell derivatives
| Autor: | Michael J. Shamblott, Joyce Axelman, Gregory O. Clark, Robert Yochem, Timothy P. Sheets, David J. Kaczorowski |
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| Rok vydání: | 2007 |
| Předmět: |
Pluripotent Stem Cells
medicine.medical_specialty medicine.medical_treatment Transplantation Heterologous Biophysics Biology Biochemistry Article Mice chemistry.chemical_compound Diabetes mellitus Internal medicine medicine Animals Humans Insulin Protein Precursors Induced pluripotent stem cell Pancreas Molecular Biology Cells Cultured Embryonic Stem Cells Type 1 diabetes C-Peptide C-peptide Cell Biology medicine.disease Embryonic stem cell Transplantation Germ Cells Glucose Endocrinology chemistry Stem cell Proinsulin Stem Cell Transplantation Transcription Factors |
| Zdroj: | Biochemical and Biophysical Research Communications. 356:587-593 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2007.03.017 |
| Popis: | Type 1 diabetes mellitus subjects millions to a daily burden of disease management, life threatening hypoglycemia and long-term complications such as retinopathy, nephropathy, heart disease, and stroke. Cell transplantation therapies providing a glucose-regulated supply of insulin have been implemented clinically, but are limited by safety, efficacy and supply considerations. Stem cells promise a plentiful and flexible source of cells for transplantation therapies. Here, we show that cells derived from human embryonic germ (EG) cells express markers of definitive endoderm, pancreatic and beta-cell development, glucose sensing, and production of mature insulin. These cells integrate functions necessary for glucose responsive regulation of preproinsulin mRNA and expression of insulin C-peptide in vitro. Following transplantation into mice, cells become insulin and C-peptide immunoreactive and produce plasma C-peptide in response to glucose. These findings suggest that EG cell derivatives may eventually serve as a source of insulin producing cells for the treatment of diabetes. |
| Databáze: | OpenAIRE |
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