Expression and immunological evaluation of the Escherichia coli-derived hepatitis C virus envelope E1 protein

Autor: Lazaro Lorenzo, Julio César Alvarez-Obregón, Juan Morales, Ivis Guerra, Dagmara Pichardo, Astrid Ramos, Santiago Dueñas-Carrera, Gillian Martínez, Odalys García, Nelson Acosta-Rivero
Rok vydání: 2000
Předmět:
Zdroj: Biotechnology and applied biochemistry. 32(2)
ISSN: 0885-4513
Popis: Immunological response against envelope protein E1 is very important in natural hepatitis C virus (HCV) infection, although it is insufficient to clear the viraemia. The HCV genomic region encoding the first 149 amino acids of the envelope E1 protein (E1(340), amino acids 192-340) was expressed in Escherichia coli (to a level of 30% of the whole cellular proteins) and purified to 85%. We measured the immune response in rabbits and mice as well as the reactivity against 37 human sera raised against the whole recombinant protein and E1-encoding peptides. From this, 51.1% of human sera were found to react with E1(340). High-level antibodies against E1(340) were obtained in rabbits and mice when immunized. These antibodies had a similar peptide-recognition pattern to that described previously for human sera. The most reactive region was located at the N-terminus of the E1 protein. Cellular immunity in mice was evaluated by delayed-type hypersensitivity assay. It revealed the induction of a CD4+ T-cell-mediated response by this protein. This E1(340) protein and the animal-derived anti-E1 sera are immunological tools that could aid in the monitoring and development of anti-HCV therapies.
Databáze: OpenAIRE