Inhibition of initiation of translation in L1210 cells by 8-azaguanine
| Autor: | D Irwin, H G Mandel, R S Rivest |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Pharmacology
Azaguanine Translation (biology) DNA Neoplasm Metabolism Cycloheximide Biology Biochemistry Molecular biology Neoplasm Proteins Mice chemistry.chemical_compound chemistry Polyribosomes Protein Biosynthesis Polysome Protein biosynthesis Animals L1210 cells 8-Azaguanine RNA Neoplasm Leukemia L1210 Eukaryotic Ribosome Cells Cultured |
| Zdroj: | Biochemical Pharmacology. 31:2505-2511 |
| ISSN: | 0006-2952 |
| DOI: | 10.1016/0006-2952(82)90061-2 |
| Popis: | The inhibition of protein synthesis by 8-azaguanine (azaG) in L1210 cells in culture was investigated. AzaG selectively inhibited protein synthesis at concentrations where viability was decreased significantly. AzaG altered the polyribosome sedimentation profile, increasing the numbers of monosomes and smaller polysemes and decreasing the number of larger polysomes. The reversal by cycloheximide of the alterations in the polysome profile suggested that azaG inhibited the initiation of translation. This was confirmed by the demonstration of inhibition of the formation of the 43S and 80S initiation complexes. |
| Databáze: | OpenAIRE |
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