Effects of capsaicin on nitric oxide synthase isoforms in prepubertal rat ovary

Autor: Berrin Zik, Korhan Altunbaş, C G Ozguden Akkoc, Şerife Tütüncü, Sabire Peker, Alper Sevimli, O Ozden
Přispěvatelé: Uludağ Üniversitesi/Veteriner Fakültesi/Histoloji-Embriyoloji Anabilim Dalı., Zik, Berrin, Peker, Sabire, Akkoç, Cansel Güzin Özgüden, F-2822-2017, AAH-9810-2021, Ondokuz Mayıs Üniversitesi
Rok vydání: 2011
Předmět:
Nitric oxide synthase type I
Rats
sprague-dawley
Expression
capsaicin
chemistry.chemical_compound
Enos
Maturation-Promoting Factor
Oocytes
NG-nitroarginine Methyl Ester
Nos2 protein
rat
Inducible nitric oxide synthase
Smooth-muscle
Progesterone
Neurons
afferent
Gonadotropin
Biotechnology & applied microbiology
biology
Innervation
General Medicine
Ovarian follicles
Immunohistochemistry
iNOS
Nitric oxide synthase
Sprague dawley rat
Medical Laboratory Technology
medicine.anatomical_structure
Nitric oxide synthase type III
eNOS
Female
Ovulation
Gene isoform
Cell biology
medicine.medical_specialty
Histology
Cells
Nerve ending
nNOS
Neuropeptide
Ovary
Article
ovarian follicles
Nitric oxide
Nos1 protein
rat
Nos3 protein
rat
Internal medicine
Substance-p
medicine
Animalia
Animals
Nerve endings
Secretion
Neuronal nitric oxide synthase
Nitric oxide synthase type II
Rattus
Animal
biology.organism_classification
Rats
Drug effect
Metabolism
Endocrinology
chemistry
Capsaicin
Localization
Enzymology
biology.protein
Rat
Endothelial nitric oxide synthase
Sensory nerve cell
Follicular development
Zdroj: Biotechnic & Histochemistry. 87:218-225
ISSN: 1473-7760
1052-0295
DOI: 10.3109/10520295.2011.608716
Popis: WOS: 000301530000009 PubMed: 21955128 Nitric oxide (NO) has emerged as an important intra-ovarian regulatory factor. We investigated effects of low dose capsaicin (CAP) treatment on the different NOS isoforms in prepubertal rat ovaries. Fifteen 21-day-old female Sprague-Dawley rats were divided randomly into three groups. The first group received no treatment, the second group received 0.5 mg/kg/day CAP dissolved in the vehicle, and the third group was treated with the vehicle only. The animals were euthanized by ether inhalation after 15 days and their ovaries were excised. Ovaries were fixed in 10% neutral buffered formalin and embedded in paraffin. Sections were processed for standard immunohistochemistry using the labeled streptavidin-biotin technique for expression of nNOS, eNOS and iNOS. We demonstrated that CAP induced expression of NOS isotypes including eNOS, iNOS and nNOS in prepubertal rat ovaries. CAP may lead to release of NO either directly from nerves or indirectly by evoking release from other cells via the action of neuropeptides that are released from afferent terminals and are involved in regulating female reproductive function. Uludag University, Bursa, TurkeyUludag University [V-2008/66] This work was supported by a grant from Uludag University Research Funds (project no: V-2008/66). Bursa, Turkey.
Databáze: OpenAIRE
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