A bioluminescent Pseudomonas aeruginosa wound model reveals increased mortality of type 1 diabetic mice to biofilm infection
| Autor: | Christopher M. Waters, Casandra L Larrivee, A.M. Agostinho Hunt, Sandra O'Reilly, Julia V. Busik, Robert B. Abramovitch, Svetlana Navitskaya, Jacob A. Gibson, David B. Needle |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Nursing (miscellaneous) 030106 microbiology medicine.disease_cause Diabetes Mellitus Experimental Microbiology Mice 03 medical and health sciences Fibrosis Diabetes mellitus Animals Medicine Bioluminescence Pseudomonas Infections In patient Wound Healing business.industry Pseudomonas aeruginosa Biofilm Diabetic mouse medicine.disease Diabetes Mellitus Type 1 Biofilms Wound Infection Fundamentals and skills Microorganisms Genetically-Modified business Wound healing |
| Zdroj: | Journal of Wound Care. 26:S24-S33 |
| ISSN: | 2052-2916 0969-0700 |
| DOI: | 10.12968/jowc.2017.26.sup7.s24 |
| Popis: | Objective: To examine how bacterial biofilms, as contributing factors in the delayed closure of chronic wounds in patients with diabetes, affect the healing process. Method: We used daily microscopic imaging and the IVIS Spectrum in vivo imaging system to monitor biofilm infections of bioluminescent Pseudomonas aeruginosa and evaluate healing in non-diabetic and streptozotocin-induced diabetic mice. Results: Our studies determined that diabetes alone did not affect the rate of healing of full-depth murine back wounds compared with non-diabetic mice. The application of mature biofilms to the wounds significantly decreased the rate of healing compared with non-infected wounds for both non-diabetic as well as diabetic mice. Diabetic mice were also more severely affected by biofilms displaying elevated pus production, higher mortality rates and statistically significant increase in wound depth, granulation/fibrosis and biofilm presence. Introduction of a mutant Pseudomonas aeruginosa capable of producing high concentrations of cyclic di-GMP did not result in increased persistence in either diabetic or non-diabetic animals compared with the wild type strain. Conclusion: Understanding the interplay between diabetes and biofilms may lead to novel treatments and better clinical management of chronic wounds. |
| Databáze: | OpenAIRE |
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