Mast cell-nerve interaction in the colon of Trypanosoma cruzi-infected individuals with chagasic megacolon
| Autor: | Enio Chaves de Oliveira, Débora d'Ávila Reis, Patrícia Rocha Martins, Rodolfo Duarte Nascimento, Patrícia Massara Martinelli, Aline Tomaz dos Santos |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Chagas disease Colon Neuroimmunomodulation Trypanosoma cruzi Tryptase Megacolon Enteric Nervous System Receptors G-Protein-Coupled 03 medical and health sciences Chymases 0302 clinical medicine Immune system parasitic diseases medicine Animals Humans Receptor PAR-2 Chagas Disease Mast Cells Aged Neurons General Veterinary biology Chymase General Medicine Middle Aged Mast cell medicine.disease biology.organism_classification Coleoptera 030104 developmental biology Infectious Diseases medicine.anatomical_structure Insect Science Immunology biology.protein Female Tryptases Parasitology Enteric nervous system 030217 neurology & neurosurgery |
| Zdroj: | Parasitology Research. 117:1147-1158 |
| ISSN: | 1432-1955 0932-0113 |
| Popis: | Chagas disease is an infection caused by the parasite Trypanosoma cruzi that affects millions of people worldwide and is endemic in Latin America. Megacolon is the most frequent complication of the digestive chronic form and happens due to lesions of the enteric nervous system. The neuronal lesions seem to initiate in the acute phase and persist during the chronic phase, albeit the mechanisms involved in this process are still debated. Among the cells of the immune system possibly involved in this pathological process is the mast cell (MC) due to its well-known role in the bi-directional communication between the immune and nervous systems. Using ultrastructural analysis, we found an increased number of degranulated MCs in close proximity to nerve fibers in infected patients when compared with uninfected controls. We also immunostained MCs for the two pro-inflammatory molecules tryptase and chymase, the first being also important in neuronal death. The number of MCs immunostained for tryptase or chymase was increased in patients with megacolon, whereas increased tryptase staining was additionally observed in patients without megacolon. Moreover, we detected the expression of the tryptase receptor PAR2 in neurons of the enteric nervous system, which correlated to the tryptase staining results. Altogether, the data presented herein point to the participation of MCs on the denervation process that occurs in the development of T. cruzi-induced megacolon. |
| Databáze: | OpenAIRE |
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