Quantitative profiling of the endonuclear glycerophospholipidome of murine embryonic fibroblasts
| Autor: | Emily K. Tribble, James G. Alb, Aby Grabon, Irene Faenza, H. Alex Brown, Lucio Cocco, Vytas A. Bankaitis, Pavlina T. Ivanova |
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| Přispěvatelé: | Tribble, Emily K, Ivanova, Pavlina T., Grabon, Aby, Alb, James G., Faenza, Irene, Cocco, LUCIO ILDEBRANDO, Brown, H. Alex, Bankaitis, Vytas A. |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell signaling Nuclear Envelope phospholipids/phosphatidylinositol QD415-436 Glycerophospholipids Biochemistry lipids phospholipids/metabolism 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Endocrinology Lipid ligands • Phospholipids/metabolism • Phosphatidylcholine medicine cell signaling Animals Phosphatidylinositol Phospholipid Transfer Proteins Cells Cultured Phospholipids Research Articles Phosphatidylethanolamine Cell Nucleus nuclear receptors/lipid ligands Chemistry Cell Biology Fibroblasts Nuclear matrix Embryo Mammalian Cell signaling • Cell biology Phospholipid Cell nucleus 030104 developmental biology medicine.anatomical_structure Nuclear receptor 030220 oncology & carcinogenesis Lipids • |
| Zdroj: | Journal of Lipid Research, Vol 57, Iss 8, Pp 1492-1506 (2016) |
| ISSN: | 1539-7262 |
| Popis: | A reliable method for purifying envelope-stripped nuclei from immortalized murine embryonic fibroblasts (iMEFs) was established. Quantitative profiling of the glycerophospholipids (GPLs) in envelope-free iMEF nuclei yields several conclusions. First, we find the endonuclear glycerophospholipidome differs from that of bulk membranes, and phosphatidylcholine (PtdCho) and phosphatidylethanolamine species are the most abundant endonuclear GPLs by mass. By contrast, phosphatidylinositol (PtdIns) represents a minor species. We also find only a slight enrichment of saturated versus unsaturated GPL species in iMEF endonuclear fractions. Moreover, much lower values for GPL mass were measured in the iMEF nuclear matrix than those reported for envelope-stripped IMF-32 nuclei. The collective results indicate that the nuclear matrix in these cells is a GPL-poor environment where GPL occupies only approximately 0.1% of the total nuclear matrix volume. This value suggests GPL accommodation in this compartment can be satisfied by binding to resident proteins. Finally, we find no significant role for the PtdIns/PtdChotransfer protein, PITPô, in shuttling PtdIns into the iMEF nuclear matrix.-Tribble, E. K., P. T. Ivanova, A. Grabon, J. G. Alb, Jr., I. Faenza, L. Cocco, H. A. Brown, and V. A. Bankaitis. Quantitative profiling of the endonuclear glycerophospholipidome of murine embryonic fibroblasts. |
| Databáze: | OpenAIRE |
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