Tobamovirus-resistant tobacco generated by RNA interference directed against host genes
| Autor: | Tetsuo Meshi, Masayuki Ishikawa, Katsuyuki Hirai, Momoko Asano, Rena Satoh, Atsuko Mochizuki, Masamichi Nishiguchi, Takuya Yamanaka, Satoshi Naito, Shinya Tsuda |
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| Rok vydání: | 2005 |
| Předmět: |
Arabidopsis thaliana
Nicotiana tabacum Resistance Molecular Sequence Data Biophysics Genes Plant Virus Replication Biochemistry Cucumber mosaic virus Structural Biology Plant virus Tobacco Genetics Tobacco mosaic virus Tomato mosaic virus Amino Acid Sequence Molecular Biology Plant Diseases biology Tobamovirus fungi food and beverages RNA Cell Biology biology.organism_classification Virology TOM1 Mutation RNA Interference Cauliflower mosaic virus |
| Zdroj: | FEBS Letters. 579:4479-4484 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/j.febslet.2005.07.021 |
| Popis: | Two homologous Nicotiana tabacum genes NtTOM1 and NtTOM3 have been identified. These genes encode polypeptides with amino acid sequence similarity to Arabidopsis thaliana TOM1 and TOM3, which function in parallel to support tobamovirus multiplication. Simultaneous RNA interference against NtTOM1 and NtTOM3 in N. tabacum resulted in nearly complete inhibition of the multiplication of Tomato mosaic virus and other tobamoviruses, but did not affect plant growth or the ability of Cucumber mosaic virus to multiply. As TOM1 and TOM3 homologues are present in a variety of plant species, their inhibition via RNA interference should constitute a useful method for generating tobamovirus-resistant plants. |
| Databáze: | OpenAIRE |
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