Real-time liver uptake and biodistribution of magnetic nanoparticles determined by AC biosusceptometry
| Autor: | Sunil Krishnan, Gustavo M. Moretto, Andris F. Bakuzis, Nicholas Zufelato, Ricardo Brandt de Oliveira, Marcos F.F. Calabresi, Oswaldo Baffa, André G. Próspero, Jose R.A. Miranda, Caio C. Quini, Diana Rodrigues de Pina |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade Federal de Goiás (UFG), Univ Texas MD Anderson Canc Ctr, Universidade de São Paulo (USP) |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Biodistribution Materials science Biomedical Engineering Analytical chemistry Pharmaceutical Science Medicine (miscellaneous) Bioengineering NANOPARTÍCULAS 02 engineering and technology 03 medical and health sciences Magnetics In vivo Distribution (pharmacology) Animals General Materials Science Tissue Distribution Nanoparticle uptake Rats Wistar Correlation index Magnetite Nanoparticles Biomedical instruments Electron Spin Resonance Spectroscopy 021001 nanoscience & nanotechnology Extravasation 030104 developmental biology AC biosusceptometry Liver Real time monitoring Magnetic nanoparticles Molecular Medicine 0210 nano-technology Quantification methods Quantitative analysis (chemistry) Ex vivo Biomedical engineering |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1549-9642 |
| Popis: | Made available in DSpace on 2018-11-26T17:34:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-05-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) We describe the development of a joint in vivo/ex vivo protocol to monitor magnetic nanoparticles in animal models. Alternating current biosusceptometry (ACB) enables the assessment of magnetic nanoparticle accumulation, followed by quantitative analysis of concentrations in organs of interest. We present a study of real-time liver accumulation, followed by the assessment of sequential biodistribution using the same technique. For quantification, we validated our results by comparing all of the data with electron spin resonance (ESR). The ACB had viable temporal resolution and accuracy to differentiate temporal parameters of liver accumulation, caused by vasculature extravasation and macrophages action. The biodistribution experiment showed different uptake profiles for different doses and injection protocols. Comparisons with the ESR system indicated a correlation index of 0.993. We present the ACB system as an accessible and versatile tool to monitor magnetic nanoparticles, allowing in vivo and real-time evaluations of distribution and quantitative assessments of particle concentrations. (C) 2017 Elsevier Inc. All rights reserved. Univ Estadual Paulista, Dept Fis & Biofis, Lab Biomagnetismo, IBB, Botucatu, SP, Brazil Univ Fed Goias, Inst Fis, Goiania, Go, Brazil Univ Texas MD Anderson Canc Ctr, Dept Expt Radiat Oncol, Houston, TX 77030 USA Univ Estadual Paulista, Fac Med Botucatu, Dept Doencas Trop & Diagnost Imagem, Botucatu, SP, Brazil Univ Sao Paulo, Fac Med Ribeirao Preto, Ribeirao Preto, SP, Brazil Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Fis, Ribeirao Preto, SP, Brazil Univ Estadual Paulista, Dept Fis & Biofis, Lab Biomagnetismo, IBB, Botucatu, SP, Brazil Univ Estadual Paulista, Fac Med Botucatu, Dept Doencas Trop & Diagnost Imagem, Botucatu, SP, Brazil FAPESP: 2010/07639-9 FAPESP: 2011/18696-6 FAPESP: 2013/20842-6 FAPESP: 2015/14914-0 |
| Databáze: | OpenAIRE |
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