ANG II stimulates endothelial nitric oxide synthase expression in bovine pulmonary artery endothelium
| Autor: | Susan C. Olson, T. A. Dowds, M. T. Barry, P. A. Pino, T. Burke-Wolin |
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| Rok vydání: | 1997 |
| Předmět: |
Pulmonary and Respiratory Medicine
medicine.medical_specialty Endothelium Physiology Pulmonary Artery chemistry.chemical_compound Physiology (medical) Internal medicine medicine Animals RNA Messenger Enzyme Inhibitors Cells Cultured Nitrites biology Angiotensin II Cell Biology medicine.disease Pulmonary hypertension Coronary Vessels Endothelial stem cell Nitric oxide synthase medicine.anatomical_structure Endocrinology chemistry biology.protein Artery Endothelium Cattle Endothelium Vascular Nitric Oxide Synthase Blood vessel Saralasin |
| Zdroj: | The American journal of physiology. 273(2 Pt 1) |
| ISSN: | 0002-9513 |
| Popis: | Although angiotensin II (ANG II) is a known pulmonary vasoconstrictor, the purpose of this study was to examine the effect of ANG II on pulmonary artery endothelial cell nitric oxide synthase (ecNOS) mRNA and protein expression. Cultured bovine pulmonary artery endothelial (BPAE; passages 5-8) cells were incubated for 0-12 h with 10(-6) M ANG II. Total RNA was extracted, and ecNOS expression was assessed by Northern blot analysis. In BPAE cells, ecNOS mRNA was significantly increased 2.4 +/- 0.3-fold (P < 0.05 vs. basal; n = 5) 6 h after the addition of ANG II over basal levels. In & similar time course, it was found that ecNOS protein concentrations are increased 247 +/- 62% (P < 0.05 vs. basal; n = 8) over basal levels 4 h after ANG II addition. There is a second protein peak 8 h after ANG II addition in which ecNOS was increased 333 +/- 145% over basal (P < 0.05, n = 3). These data suggest that ANG II stimulates ecNOS mRNA expression and are followed by increased levels of ecNOS protein in cultured BPAE cells, consistent with an observed increase in nitrite production. Both the increase in ecNOS protein and mRNA expression could be inhibited with the ANG II receptor antagonist saralasin. Additionally, actinomycin D, an inhibitor of transcription, prevented the rise in mRNA at 6 h while cycloheximide inhibited the initial protein peak. The effects of ANG II on ecNOS were specific for the pulmonary artery endothelium. Addition of ANG II did not increase ecNOS protein or mRNA expression in parallel studies in bovine coronary artery endothelium. The stimulation of ecNOS by ANG II may act to protect the lung and maintain low pulmonary artery pressures in the renin-angiotensin model of systemic hypertension. |
| Databáze: | OpenAIRE |
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