Heterogeneity of colorectal adenocarcinomas evaluated by flow cytometry and histopathology
| Autor: | Philip Quirke, Colin C. Bird, J. E. D. Dyson, C. A. F. Joslin, Michael F. Dixon |
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| Rok vydání: | 1985 |
| Předmět: |
Cancer Research
medicine.medical_specialty Pathology Cell Survival Rectum Adenocarcinoma Biology Flow cytometry chemistry.chemical_compound medicine Humans Stage (cooking) Grading (tumors) Neoplasm Staging Ploidies Staining and Labeling medicine.diagnostic_test Rectal Neoplasms Acridine orange DNA Neoplasm Flow Cytometry medicine.disease Staining medicine.anatomical_structure Oncology chemistry Colonic Neoplasms Histopathology Research Article |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| DOI: | 10.1038/bjc.1985.14 |
| Popis: | Flow cytometry and histopathology were utilised in evaluating 50 primary and 16 metastatic colorectal carcinomas to determine the influence of heterogeneity and proportion of dying cells on pathological assessments. A new procedure was developed for staining unfixed whole cells with acridine orange and ethidium bromide to quantify DNA and RNA content and number of dead and dying cells. Attempts were made to reduce interobserver variation in histological assessment and to determine whether flow cytometry could refine current grading and staging procedures. Interobserver variation in grading was not improved by estimating proportions of differing grades in multiple samples from individual tumours. Considerable heterogeneity was observed within tumours although this was less apparent when defining ploidy status than histological grade. No consistent differences were observed between superficial and deep parts of tumours or between primary and secondary tumours by either method of analysis. The proportion of dead and dying cells varied widely between tumours but there was no correlation with tumour grade or stage. Non-diploid tumours were not of more advanced stage or poorer histological grade than diploid tumours. Since ploidy status may be an important prognostic factor, analysis of colorectal carcinomas by flow cytometry could be of greater value than conventional grading and staging procedures. Images Figure 3 |
| Databáze: | OpenAIRE |
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