Structural Diversity and Highly Specific Host-Pathogen Transcriptional Regulation of Defensin Genes Is Revealed in Tomato
| Autor: | Nikolaos Nikoloudakis, Polyxeni Pappi, Costas Delis, Georgios Tsaniklidis, Emmanouil A. Markakis, Emmanuel A. Tzortzakakis, Dimitrios Fanourakis, Konstantinos A. Paschalidis, Spyridoula N. Charova |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Nematodes tomato Verticillium 01 natural sciences PVY lcsh:Chemistry Defensins Cucumber mosaic virus Biotic stress Solanum lycopersicum Gene Expression Regulation Plant Transcriptional regulation lcsh:QH301-705.5 Defensin Spectroscopy Plant Proteins Genetics Meloidogyne javanica Agricultural Sciences Verticillium wilt CMV food and beverages General Medicine Computer Science Applications Potato virus Y Host-Pathogen Interactions Cold stress Transcriptional Activation Plant defensin Biology Tomato Article Catalysis Inorganic Chemistry 03 medical and health sciences biotic stress Animals Tylenchoidea Verticillium dahliae Physical and Theoretical Chemistry Molecular Biology Gene Cold-Shock Response fungi Organic Chemistry Agriculture Forestry and Fisheries biology.organism_classification 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 nematodes cold stress 010606 plant biology & botany |
| Zdroj: | International Journal of Molecular Sciences Volume 21 Issue 24 International Journal of Molecular Sciences, Vol 21, Iss 9380, p 9380 (2020) |
| ISSN: | 1422-0067 |
| Popis: | Defensins are small and rather ubiquitous cysteine-rich anti-microbial peptides. These proteins may act against pathogenic microorganisms either directly (by binding and disrupting membranes) or indirectly (as signaling molecules that participate in the organization of the cellular defense). Even though defensins are widespread across eukaryotes, still, extensive nucleotide and amino acid dissimilarities hamper the elucidation of their response to stimuli and mode of function. In the current study, we screened the Solanum lycopersicum genome for the identification of defensin genes, predicted the relating protein structures, and further studied their transcriptional responses to biotic (Verticillium dahliae, Meloidogyne javanica, Cucumber Mosaic Virus, and Potato Virus Y infections) and abiotic (cold stress) stimuli. Tomato defensin sequences were classified into two groups (C8 and C12). Our data indicate that the transcription of defensin coding genes primarily depends on the specific pathogen recognition patterns of V. dahliae and M. javanica. The immunodetection of plant defensin 1 protein was achieved only in the roots of plants inoculated with V. dahliae. In contrast, the almost null effects of viral infections and cold stress, and the failure to substantially induce the gene transcription suggest that these factors are probably not primarily targeted by the tomato defensin network. |
| Databáze: | OpenAIRE |
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