Non-neuronal expression of SARS-CoV-2 entry genes in the olfactory system suggests mechanisms underlying COVID-19-associated anosmia
| Autor: | Sandrine Dudoit, Rebecca K. Chance, Elizabeth Purdom, Darren W. Logan, Iain C. Macaulay, Boying Gong, Ralph Abi Hachem, Koen Van den Berge, Jonathan Mill, Hiroaki Matsunami, Tatsuya Tsukahara, Diya Das, Hector Roux de Bézieux, Davide Risso, Hsin-jung Chou, Caleb Weinreb, Russell B. Fletcher, Matthew S. Grubb, John Ngai, David H. Brann, Marcela Lipovsek, Sandeep Robert Datta, Bradley J. Goldstein, Kelly Street, Yoon Gi Choi |
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| Jazyk: | angličtina |
| Předmět: |
Olfactory system
VARIANT DIVERSITY Mice Olfaction Disorders 0302 clinical medicine RESPIRATORY SYNDROME CORONAVIRUS FUNCTIONAL RECEPTOR Medicine and Health Sciences NEURON 0303 health sciences Multidisciplinary Serine Endopeptidases Callithrix STEM Smell Mathematics and Statistics medicine.anatomical_structure Angiotensin-Converting Enzyme 2 medicine.symptom Coronavirus Infections Cell type EPITHELIUM Pneumonia Viral Anosmia Sensory system Olfaction Peptidyl-Dipeptidase A Biology Olfactory Receptor Neurons Betacoronavirus 03 medical and health sciences Olfactory mucosa Olfactory Mucosa SARS CORONAVIRUS medicine Animals Humans Pandemics 030304 developmental biology SARS-CoV-2 COVID-19 Virus Internalization Olfactory bulb nervous system CELLS Macaca RNA-SEQ DATA MOUSE HEPATITIS-VIRUS Olfactory epithelium Neuroscience BULB LINE 030217 neurology & neurosurgery |
| Zdroj: | Science Advances Brann, D H, Tsukahara, T, Weinreb, C, Lipovsek, M, Van den Berge, K, Gong, B, Chance, R, Macaulay, I C, Chou, H J, Fletcher, R B, Das, D, Street, K, de Bezieux, H R, Choi, Y G, Risso, D, Dudoit, S, Purdom, E, Mill, J, Hachem, R A, Matsunami, H, Logan, D W, Goldstein, B J, Grubb, M S, Ngai, J & Datta, S R 2020, ' Non-neuronal expression of SARS-CoV-2 entry genes in the olfactory system suggests mechanisms underlying COVID-19-associated anosmia ', Science Advances, vol. 6, no. 31, abc5801 . https://doi.org/10.1126/sciadv.abc5801 SCIENCE ADVANCES |
| ISSN: | 2375-2548 |
| DOI: | 10.1126/sciadv.abc5801 |
| Popis: | Altered olfactory function is a common symptom of COVID-19, but its etiology is unknown. A key question is whether SARS-CoV-2 (CoV-2) – the causal agent in COVID-19 – affects olfaction directly, by infecting olfactory sensory neurons or their targets in the olfactory bulb, or indirectly, through perturbation of supporting cells. Here we identify cell types in the olfactory epithelium and olfactory bulb that express SARS-CoV-2 cell entry molecules. Bulk sequencing demonstrated that mouse, non-human primate and human olfactory mucosa expresses two key genes involved in CoV-2 entry, ACE2 and TMPRSS2. However, single cell sequencing revealed that ACE2 is expressed in support cells, stem cells, and perivascular cells, rather than in neurons. Immunostaining confirmed these results and revealed pervasive expression of ACE2 protein in dorsally-located olfactory epithelial sustentacular cells and olfactory bulb pericytes in the mouse. These findings suggest that CoV-2 infection of non-neuronal cell types leads to anosmia and related disturbances in odor perception in COVID-19 patients. |
| Databáze: | OpenAIRE |
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