Exercise Training Ameliorates Cognitive Dysfunction in Amyloid Beta-injected Rat Model: Possible Mechanisms of Angiostatin/VEGF Signaling
| Autor: | Hamid Rajabi, Reza Gharakhanlu, Yoonjung Park, Aliasghar Zarezadehmehrizi, Jonghae Lee, Junyoung Hong, Mohammad Azimi, Naser Naghdi |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A 0301 basic medicine medicine.medical_specialty Amyloid beta Morris water navigation task Hippocampus Biochemistry 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound 0302 clinical medicine Alzheimer Disease Internal medicine medicine Animals Hippocampus (mythology) Cognitive Dysfunction Rats Wistar Maze Learning Angiostatins Cognitive deficit Amyloid beta-Peptides Angiostatin biology business.industry Neurogenesis Kinase insert domain receptor Rats Vascular endothelial growth factor Disease Models Animal 030104 developmental biology Endocrinology chemistry biology.protein Neurology (clinical) medicine.symptom business 030217 neurology & neurosurgery |
| DOI: | 10.21203/rs.3.rs-214291/v1 |
| Popis: | Vascular endothelial growth factor (VEGF) regulates angio/neurogenesis and also tightly links to the pathogenesis of Alzheimer's disease (AD). Although exercise has a beneficial effect on neurovascular function and cognitive function, the direct effect of exercise on VEGF-related signaling and cognitive deficit in AD is incompletely understood. Therefore, the purpose of this study was to investigate the protective effect of exercise on angiostatin/VEGF cascade and cognitive function in AD model rats. Wistar male rats were randomly divided into five groups: control (CON), injection of DMSO (Sham-CON), CON-exercise (sham-EX), intrahippocampal injection of Aβ (Aβ), and Aβ-exercise (Aβ-EX). Rats in EX groups underwent treadmill exercise for 4 weeks, then the cognitive function was measured by the Morris Water Maze (MWM) test. mRNA levels of hypoxia-induced factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor 2 (VEGFR2), and angiostatin were determined in hippocampus by RT-PCR. We found that spatial learning and memory were impaired in Aβ-injected rats, but exercise training improved it. Moreover, exercise training increased the reduced mRNA expression level of VEGF signaling, including HIF1α, VEGF, and VEGFR2 in the hippocampus from Aβ-injected rats. Also, the mRNA expression level of angiostatin was elevated in the hippocampus from Aβ-injected rats, and exercise training abrogated its expression. Our findings suggest that exercise training improves cognitive function in Aβ-injected rats, possibly through enhancing VEGF signaling and reducing angiostatin. |
| Databáze: | OpenAIRE |
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