Enzymatically Inactive Eosinophil Peroxidase Inhibits Proinflammatory Cytokine Transcription and Secretion by Macrophages
| Autor: | Robert C. Allen, John A. Lincoln, Doris L. Lefkowitz, Ken Grattendick, Stanley S. Lefkowitz |
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| Rok vydání: | 1999 |
| Předmět: |
Male
Eosinophil Peroxidase Transcription Genetic medicine.medical_treatment Immunology Proinflammatory cytokine Mice medicine Animals RNA Messenger Interleukin 6 Inflammation biology Interleukin-6 Tumor Necrosis Factor-alpha Macrophages Interferon-alpha Interferon-beta Eosinophil Molecular biology Eosinophils Mice Inbred C57BL medicine.anatomical_structure Cytokine Peroxidases Myeloperoxidase biology.protein Cytokines Cattle Electrophoresis Polyacrylamide Gel Female Cytokine secretion Tumor necrosis factor alpha Eosinophil peroxidase Interleukin-1 |
| Zdroj: | Cellular Immunology. 196:23-33 |
| ISSN: | 0008-8749 |
| Popis: | The present investigators have reported previously that macrophages (Mphi) can bind either myeloperoxidase (MPO) or eosinophil peroxidase (EPO) resulting in enhanced cytotoxicity to Candida albicans. Since MPO was shown to be immunomodulatory, the present study was initiated to determine whether either EPO or partially fragmented EPO (fgEPO) also modulated cytokine secretion. Murine peritoneal Mphi; simultaneously stimulated with fgEPO and one of the following, (1) LPS, (2) mannosylated bovine serum albumin (mBSA), (3) interferon-gamma (IFN-gamma), or (4) Poly I:C, demonstrated both dose- and time-dependent decreases in TNF-alpha and IL-6 and a dose-dependent decrease in IFN-alpha/beta. The mRNA levels of Mphi exposed to fgEPO and mBSA demonstrated that fgEPO modulated Mphi cytokine function by decreasing TNF-alpha and IL-6 mRNA transcripts without altering transcription of TGF-beta or GM-CSF. These results demonstrate a possible interaction between the Mphi and eosinophil that could result in reduction of inflammation. |
| Databáze: | OpenAIRE |
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