A generic model for diffusive dynamics of the substrate and fluorescein tagged enzyme in microfluidic platform
| Autor: | Ece Yildiz-Ozturk, Ozlem Yesil-Celiktas, Mesut Yucel |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
chemistry.chemical_classification
Materials science Microchannel 010401 analytical chemistry Microfluidics 02 engineering and technology 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences 0104 chemical sciences Electronic Optical and Magnetic Materials Volumetric flow rate chemistry.chemical_compound Enzyme chemistry Chemical engineering Hardware and Architecture Homogeneity (physics) Self-healing hydrogels Electrical and Electronic Engineering Fluorescein 0210 nano-technology Transport phenomena |
| Zdroj: | Microsystem Technologies. 24:3095-3105 |
| ISSN: | 1432-1858 0946-7076 |
| Popis: | The aim of this study was to develop a model that describes enzymatic conversion in a microfluidic system along with convective and diffusive transport of substrate/product within the microchannels. Hence, a 1-D partial differential equation model based on Fick's Law was employed in order to describe the substrate/product concentration distributions within the system. Enzyme immobilized in PEO-inclusive/non-inclusive TEOS-based hydrogels were tagged with fluorescein and examined under confocal laser scanning microscopy to evaluate the distribution of the enzyme. The image processing analysis demonstrated that 88.2 +/- 5.9% of the PEO-inclusive gel fragment surface (pixelated) area was occupied by the enzyme-dye complex with an emission density of 29.92 +/- 1.07. Subsequent to ensuring the homogeneity throughout the microchannel, the effects of flow rate and the distance for a range of inlet substrate concentrations were considered in regards to various substrate concentrations and conversion rates. The developed model provided a quantification for the conversion of substrate into products and enhanced understanding of the transport phenomena in the hydrogel. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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