Eicosapentaenoic acid inhibits intestinal β-carotene absorption by downregulation of lipid transporter expression via PPAR-α dependent mechanism
| Autor: | Sudip Ghosh, Ravindranadh Palika, Palsa Kondaiah, Madhavan K. Nair, Pullakhandam Raghu, Purna Chandra Mashurabad |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Biophysics Peroxisome proliferator-activated receptor Down-Regulation Biology Biochemistry 03 medical and health sciences Downregulation and upregulation Humans PPAR alpha Scavenger receptor Intestinal Mucosa Molecular Biology Transcription factor Carotenoid chemistry.chemical_classification Dose-Response Relationship Drug Transporter Scavenger Receptors Class B Lipid Metabolism beta Carotene Eicosapentaenoic acid Intestines 030104 developmental biology chemistry Eicosapentaenoic Acid Intestinal Absorption Docosahexaenoic acid lipids (amino acids peptides and proteins) Caco-2 Cells Signal Transduction |
| Zdroj: | Archives of biochemistry and biophysics. 590 |
| ISSN: | 1096-0384 |
| Popis: | The involvement of lipid transporters, the scavenger receptor class B, type I (SR-BI) and Niemann-Pick type C1 Like 1 protein (NPC1L1) in carotenoid absorption is demonstrated in intestinal cells and animal models. Dietary ω-3 fatty acids are known to possess antilipidemic properties, which could be mediated by activation of PPAR family transcription factors. The present study was conducted to determine the effect of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), on intestinal β-carotene absorption. β-carotene uptake in Caco-2/TC7 cells was inhibited by EPA (p 0.01) and PPARα agonist (P 0.01), but not by DHA, PPARγ or PPARδ agonists. Despite unaltered β-carotene uptake, both DHA and PPARδ agonists inhibited the NPC1L1 expression. Further, EPA also induced the expression of carnitine palmitoyl transferase 1A (CPT1A) expression, a PPARα target gene. Interestingly, EPA induced inhibition of β-carotene uptake and SR B1 expression were abrogated by specific PPARα antagonist, but not by PPARδ antagonist. EPA and PPARα agonist also inhibited the basolateral secretion of β-carotene from Caco-2 cells grown on permeable supports. These results suggest that EPA inhibits intestinal β-carotene absorption by down regulation of SR B1 expression via PPARα dependent mechanism and provide an evidence for dietary modulation of intestinal β-carotene absorption. |
| Databáze: | OpenAIRE |
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