Inflammatory and lipid regulation by cholinergic activity in epicardial stromal cells from patients who underwent open‐heart surgery

Autor: Marinela Couselo-Seijas, Darío Durán-Muñoz, Adriana Rozados-Luis, Sonia Eiras, Laila González-Melchor, J N Lopez-Canoa, José Ramón González-Juanatey, Luisa M. Seoane, Ángel L. Fernández, Moisés Rodríguez-Mañero
Rok vydání: 2020
Předmět:
0301 basic medicine
Neutrophils
Vagotomy
Calcium in biology
Rats
Sprague-Dawley
0302 clinical medicine
Atrial Fibrillation
Adipocytes
Cells
Cultured
Chemokine CCL2
Metabolic Syndrome
parasympathetic dysfunction
Atrial fibrillation
Middle Aged
Blood proteins
Chemotaxis
Leukocyte
Adipose Tissue
030220 oncology & carcinogenesis
Molecular Medicine
Original Article
medicine.symptom
Pericardium
Acetylcholine
medicine.drug
medicine.medical_specialty
Stromal cell
epicardial fat
Subcutaneous Fat
HL-60 Cells
Inflammation
Fatty Acid-Binding Proteins
03 medical and health sciences
Parasympathetic Nervous System
medicine
Animals
Humans
Calcium Signaling
Obesity
Cardiac Surgical Procedures
Aged
business.industry
Gene Expression Profiling
Original Articles
Cell Biology
Lipid Metabolism
medicine.disease
Rats
Surgery
030104 developmental biology
Cholinergic
Stromal Cells
business
Ex vivo
Zdroj: Journal of Cellular and Molecular Medicine
ISSN: 1582-4934
1582-1838
Popis: The modulation of acetylcholine (ACh) release by botulinum toxin injection into epicardial fat diminishes atrial fibrillation (AF) recurrence. These results suggest an interaction between autonomic imbalance and epicardial fat as risk factors of AF. Our aim was to study the inflammatory, lipidic and fibroblastic profile of epicardial stroma from patients who underwent open‐heart surgery, their regulation by cholinergic activity and its association with AF. We performed in vitro and ex vivo assays from paired subcutaneous and epicardial stromal cells or explants from 33 patients. Acute ACh effects in inflammation and lipid‐related genes were analysed by qPCR, in intracellular calcium mobilization were performed by Fluo‐4 AM staining and in neutrophil migration by trans‐well assays. Chronic ACh effects on lipid accumulation were visualized by AdipoRed. Plasma protein regulation by parasympathetic denervation was studied in vagotomized rats. Our results showed a higher pro‐inflammatory profile in epicardial regarding subcutaneous stromal cells. Acute ACh treatment up‐regulated monocyte chemoattractant protein 1 levels. Chronic ACh treatment improved lipid accumulation within epicardial stromal cells (60.50% [22.82‐85.13] vs 13.85% [6.17‐23.16], P
Databáze: OpenAIRE
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