Galectin-1 modulates human melanoma cell adhesion to laminin
| Autor: | Mark E. Sobel, F. Van Den Brule, Vincenzo Castronovo, Crina Buicu, M. Baldet, P. Marschal, Douglas N.W. Cooper |
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| Rok vydání: | 1995 |
| Předmět: |
animal structures
Galectin 1 Cell Biophysics Fluorescent Antibody Technique In Vitro Techniques Biochemistry Laminin Lectins otorhinolaryngologic diseases medicine Cell Adhesion Tumor Cells Cultured Humans Cell adhesion Molecular Biology Galectin biology Cell adhesion molecule Melanoma Cell Biology medicine.disease Cell biology stomatognathic diseases medicine.anatomical_structure Hemagglutinins Cell culture Galectin-1 biology.protein Cell Adhesion Molecules |
| Zdroj: | Biochemical and biophysical research communications. 209(2) |
| ISSN: | 0006-291X |
| Popis: | Galectins constitute a gene family of beta-galactoside-specific lectins that show high homology in their carbohydrate-binding site. They have been postulated to be involved in many biological events, but their specific functions are not yet well defined. Galectin-1 is a laminin binding protein that recognizes poly-N-acetyllactosamine chains on this major basement membrane glycoprotein. In this study, we analyzed the possibility that galectin-1 could modulate interactions between human melanoma cells and laminin. We demonstrated that A375 and A2058 cell lines express galectin-1 both intracellularly and on the cell surface. In an in vitro assay, recombinant galectin-1 increased melanoma cell attachment to laminin in a dose-dependent manner. This effect was abolished by lactose. Anti-galectin-1 inhibited adhesion of melanoma cells to laminin in a dose-dependent fashion. However, neither galectin-1 nor anti-galectin-1 antibody affected melanoma cell spreading on laminin in vitro. These data indicate that galectin-1 might participate in melanoma cell adhesion to laminin and therefore could be a modulator of invasion and metastasis. |
| Databáze: | OpenAIRE |
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