Localization of the 70000 Dalton Heat-induced protein in the nuclear matrix of BHK cells
| Autor: | E. St-Pierre, V. Bibor-Hardy, Marcel Pouchelet, R. Simard |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Cell Nucleus
Cytoplasm Hot Temperature HSPA14 Isoelectric focusing DNA Cell Biology Biology Nuclear matrix Cell Line Molecular Weight HSPA4 medicine.anatomical_structure Biochemistry Cell culture Cricetinae Heat shock protein medicine Biophysics Animals Electrophoresis Polyacrylamide Gel Isoelectric Focusing Nucleus Heat-Shock Proteins |
| Zdroj: | Experimental Cell Research. 149:451-459 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/0014-4827(83)90357-9 |
| Popis: | The exposure of exponentially growing BHK cells to supranormal temperatures (41-44 degrees C, for 15 min to 1 h) induces the synthesis of a new set of proteins, the heat shock proteins, while the synthesis of proteins made before heat shock is repressed at 43 degrees C. Among the two major heat shock proteins induced, of molecular weight 70 K and 68 K, only the 70 kD protein is found bound to the nuclear matrix. This protein is resolved differently from the normal matrix proteins by isoelectric focusing and, when blotted, does not react with antibodies directed against nuclear matrices. These results show that the 70 kD heat shock protein is a new protein transferred from the cytoplasm to the nucleus, where it binds to the nuclear matrix, suggesting a structural role for this protein. |
| Databáze: | OpenAIRE |
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