Prophylactic effects of biogenic selenium nanoparticles on acute toxoplasmosis: An in vivo study
| Autor: | Hossein Mahmoudvand, Esraa Gabal, Sareh Jahanbakhsh, Ebrahim Badparva, Mojtaba Shakibaie, Fatemeh Ezzatkhah |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_treatment
Toxoplasma gondii Pharmacology Parasite load Prophylactic 03 medical and health sciences Selenium 0302 clinical medicine In vivo Medicine Saline Original Research biology Toxicity business.industry General Medicine biology.organism_classification medicine.disease Toxoplasmosis Nitric oxide synthase Treatment 030220 oncology & carcinogenesis biology.protein Interleukin 12 030211 gastroenterology & hepatology Surgery business |
| Zdroj: | Annals of Medicine and Surgery |
| ISSN: | 2049-0801 |
| Popis: | Background In this investigation, the in vivo efficacy and safety of biogenic selenium nanoparticles (SeNPs) are assessed against acute toxoplasmosis caused by Toxoplasma gondii (Sarcocystidae) in the mice. Methods Male NMRI mice were orally treated with normal saline (control group) and SeNPs at the doses of 5 and 10 mg/kg once a day for 14 days. On the 15th day, the mice were infected with 104 tachyzoites of T. gondii RH strain by the intraperitoneal route. The mortality rate and parasite load were determined in the infected mice. The mRNA levels of IFN-γ, IL10, IL12, and inducible nitric oxide synthase were also examined in the infected mice by quantitative real-time PCR. Results The rate of mortality in the infected mice receiving SeNPs at the doses of 5 and 10 mg/kg compared with the mice in the control group was 100% on the 9 and 10 days after the administration. The mean number of tachyzoites in the infected mice receiving SeNPs was significantly lower than that in the control group. No significant difference (p > 0.05) was found in the biochemical parameters between the mice treated with SeNPs and the mice in the control group. The results revealed that mRNA levels significantly improved in the infected mice treated with SeNPs compared with those in the control group. Conclusion Findings of the present investigation showed the considerable efficacy of SeNPs with no important toxicity for curing acute toxoplasmosis in the mice model. However, further studies are needed to clarify the accurate anti-Toxoplasma mechanisms of SeNPs. Highlights • We evaluated the efficacy of Biogenic Selenium Nanoparticles (SeNPs) against acute toxoplasmosis. • Mice were orally treated with SeNPs at the doses of 5 and 10 mg/kg once a day for 14 days. • The average number of tachyzoites and mortality rate was significantly lower in treated mice. • No significant difference (p > 0.05) observed in the biochemical parameters between the mice treated. • The mRNA levels of meaningfully improved in infected mice treated with SeNPs. |
| Databáze: | OpenAIRE |
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