Sorting nexin 9 (SNX9) is not essential for development and auditory function in mice
| Autor: | Chengcheng Liu, Huiren Cao, Yujie Cao, Zhigang Xu, Haibo Du, Yanfei Wang, Jiangang Gao, Xiaoyan Zhai, Xiao Yu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male inner ear hair cells Endosome Sorting Nexins Biology 03 medical and health sciences Mice 0302 clinical medicine Hearing Hair Cells Auditory medicine Animals Inner ear RNA Messenger Homologous Recombination Actin Mice Knockout Membrane tubulation Behavior Animal Gene Expression Regulation Developmental Actins Endocytosis Cell biology Sorting nexin 030104 developmental biology medicine.anatomical_structure Oncology Immunology Knockout mouse Female Noise Developmental biology SNX9 030217 neurology & neurosurgery knockout mice Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | // Chengcheng Liu 1 , Xiaoyan Zhai 1 , Haibo Du 1 , Yujie Cao 1, 3 , Huiren Cao 1 , Yanfei Wang 1 , Xiao Yu 2 , Jiangang Gao 1 , Zhigang Xu 1 1 Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong 250100, P. R. China. 2 Department of Physiology, Shandong University School of Medicine, Jinan, Shandong 250012, P. R. China 3 Current Address: Cell Biology, Department of Biology, Faculty of Science, Utrecht University, CH Utrecht 3584, The Netherlands Correspondence to: Zhigang Xu, email: xuzg@sdu.edu.cn Keywords: SNX9, knockout mice, inner ear, hearing, hair cells Received: April 07, 2016 Accepted: September 02, 2016 Published: September 15, 2016 ABSTRACT Sorting nexins are a large family of evolutionarily conserved proteins that play fundamental roles in endocytosis, endosomal sorting and signaling. As an important member of sorting nexin family, sorting nexin 9 (SNX9) has been shown to participate in coordinating actin polymerization with membrane tubulation and vesicle formation. We previously showed that SNX9 is expressed in mouse auditory hair cells and might regulate actin polymerization in those cells. To further examine the physiological role of SNX9, we generated Snx9 knockout mice using homologous recombination method. Unexpectedly, Snx9 knockout mice have normal viability and fertility, and are morphologically and behaviorally indistinguishable from control mice. Further investigation revealed that the morphology and function of auditory hair cells are not affected by Snx9 inactivation, and Snx9 knockout mice have normal hearing threshold. In conclusion, our data revealed that Snx9 -deficient mice do not show defects in development as well as auditory function, suggesting that SNX9 is not essential for mice development and hearing. |
| Databáze: | OpenAIRE |
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