Aberrant c-erbB2 expression in cell clusters overlying focally disrupted breast myoepithelial cell layers: a trigger or sign for emergence of more aggressive cell clones?
| Autor: | Chunling Gao, Joy Sheng, Jeffrey T. Mason, Pengtao Gong, Xichen Zhang, Shahreyar Shar Hashemi, Jinsong Ni, Qiuyue Wang, Yan-gao Man, Ling Gao, Morvarid Yousefi |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Receptor
ErbB-2 Tumor invasion Cell Chromogenic in situ hybridization Breast Neoplasms In situ hybridization Biology Applied Microbiology and Biotechnology myoepithelial cells Stroma Gene expression tumor stem cells medicine Humans Neoplasm Invasiveness c-erbB2 Molecular Biology Ecology Evolution Behavior and Systematics In Situ Hybridization Reverse Transcriptase Polymerase Chain Reaction Myoepithelial cell Epithelial Cells Cell Biology Molecular biology Immunohistochemistry monoclonal proliferation Gene Expression Regulation Neoplastic medicine.anatomical_structure Carcinoma Intraductal Noninfiltrating Cytoplasm Immunology Disease Progression Female Developmental Biology Research Paper |
| Zdroj: | International Journal of Biological Sciences |
| ISSN: | 1449-2288 |
| Popis: | Our recent studies revealed that cell clusters overlying focal myoepithelial cell layer disruption (FMCLD) had a significantly higher frequency of genetic instabilities and expression of invasion-related genes than their adjacent counterparts within the same duct. Our current study attempted to assess whether these cell clusters would also have elevated c-erbB2 expression. Human breast tumors (n=50) with a high frequency of FMCLD were analyzed with double immunohistochemistry, real-time RT-PCR, and chromogenic in situ hybridization for c-erbB2 protein and gene expression. Of 448 FMCLD detected, 404 (90.2%) were associated with cell clusters that had intense c-erbB2 immunoreactivities primarily in their cytoplasm, in contrast to their adjacent counterparts within the same duct, which had no or barely detectable c-erbB2 expression. These c-erbB2 positive cells were arranged as tongue-like projections, "puncturing" into the stroma, and about 20% of them were in direct continuity with tube-like structures that resembled blood vessels. Aberrant c-erbB2 expression was also seen in clusters of architecturally normal-appearing ducts that had distinct cytological abnormalities in both ME and epithelial cells, whereas not in their clear-cut normal counterparts. Molecular assays detected markedly higher c-erbB2 mRNA and gene amplification in cell clusters associated with FMCLD than in those associated with non-disrupted ME cell layers. Our findings suggest that cell clusters overlying FMCLD may represent the precursors of pending invasive lesions, and that aberrant c-erbB2 expression may trigger or signify the emergence of biologically more aggressive cell clones. |
| Databáze: | OpenAIRE |
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