Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci
| Autor: | José Maurício Barbanti Duarte, Maurício Durante Christofoletti, Renato Caparroz, Aline Meira Bonfim Mantellatto, Ubiratan Piovezan |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade de Brasília (UnB), Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine fecal DNA lcsh:QH426-470 Population Zoology Pampas deer Locus (genetics) Conservation Biology 010603 evolutionary biology 01 natural sciences microsatellites 03 medical and health sciences Genetics Genetic variability Allele education Molecular Biology education.field_of_study Genetic diversity Cervo-do-pantanal Ecology Conservação biology.organism_classification non-invasive methods lcsh:Genetics 030104 developmental biology pampas deer Threatened species Microsatellite Animal Genetics Microssatélites |
| Zdroj: | Genetics and Molecular Biology, Iss 0 (2017) Genetics and Molecular Biology v.40 n.4 2017 Genetics and Molecular Biology Sociedade Brasileira de Genética (SBG) instacron:SBG Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Genetics and Molecular Biology, Volume: 40, Issue: 4, Pages: 774-780, Published: 02 OCT 2017 Genetics and Molecular Biology, Issue: ahead, Published: 02 OCT 2017 Repositório Institucional da UnB Universidade de Brasília (UnB) instacron:UNB |
| ISSN: | 1678-4685 1415-4757 |
| Popis: | Made available in DSpace on 2018-12-11T17:35:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-10-01. Added 1 bitstream(s) on 2021-07-15T15:04:25Z : No. of bitstreams: 1 S1415-47572017000500774.pdf: 977503 bytes, checksum: 1dc9207855ab8173e7b740255fa226cb (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The pampas deer (Ozotoceros bezoarticus) is close to being classified as globally threatened’, with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to evaluate the genetic variability of the Brazilian Pantanal population of pampas deer. Six heterologous microsatellite markers were used to screen 142 stool specimens. Seventy-four deer were identified, of which 50 adults were used to determine the genetic characteristics of the population. The Pantanal population showed high genetic diversity (mean number of alleles per locus = 11.5, expected heterozygosity = 0.75). This is the first investigation to characterize a South American deer species using fecal DNA and demonstrates the usefulness and efficiency of this approach, as well as the feasibility of obtaining information that could not have been easily obtained by traditional DNA sampling. Our findings suggest that management strategies for this species may be much more effective if applied now when the population still shows high genetic variability. Núcleo de Pesquisa e Conservação de Cervídeos Departamento de Zootecnia Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) Departamento de Genética e Morfologia Instituto de Ciências Biológicas Universidade de Brasília Centro de Pesquisas Agropecuárias do Pantanal (CPAP), Embrapa Núcleo de Pesquisa e Conservação de Cervídeos Departamento de Zootecnia Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) FAPESP: 2009/04253-5 |
| Databáze: | OpenAIRE |
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