Characterization of the canine type C enterotoxin produced by Staphylococcus intermedius pyoderma isolates
| Autor: | Gregory A. Bohach, Philip H. Berger, Claudia F. Deobald, James R. Deringer, Vincent M. Edwards, Scott D. Callantine, Vivek Kapur, Cynthia V. Stauffacher |
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| Rok vydání: | 1997 |
| Předmět: |
Cellular immunity
Receptors Antigen T-Cell alpha-beta Staphylococcus Molecular Sequence Data Immunology Sequence alignment Enterotoxin medicine.disease_cause Microbiology Enterotoxins Dogs medicine Superantigen Animals Humans Amino Acid Sequence Dog Diseases Peptide sequence Alleles Base Sequence biology Staphylococcus intermedius biology.organism_classification Infectious Diseases Pyoderma Staphylococcus aureus Parasitology Macaca nemestrina Research Article |
| Zdroj: | Infection and Immunity. 65:2346-2352 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.65.6.2346-2352.1997 |
| Popis: | The type C staphylococcal enterotoxins (SECs) are a group of highly conserved proteins with substantial antigenic cross-reactivity. Although Staphylococcus intermedius and coagulase-positive species of staphylococci are reported to produce SEC and other SEs, toxins produced by species other than Staphylococcus aureus have not been previously characterized. In this study we report the molecular, biological, and immunological properties of the canine SEC (SECcanine) expressed by pathogenic isolates of S. intermedius. The mature form of SECcanine has 239 amino acid residues and a pI of 7.0. Typical of the SEs, purified SECcanine induces an emetic response in monkeys and the proliferation of T cells in a Vbeta-dependent manner. Although SECcanine has >95% sequence identity to previously described SEC variants, its sequence is most related to SEC2 and SEC3. In contrast to the sequence similarity, the Vbeta profile induced by SECcanine is typical of that induced by SEC1. This result is likely explained by the conservation of a cysteine residue at position 26 in SECcanine; residues at this position have been previously shown to determine subtype-dependent differences in T-cell receptor interactions of other SEs. Overall, these results show that superantigen toxins produced by the multiple members of the genus Staphylococcus are highly conserved in respect to biological and structural properties. Further, the frequent association of SECcanine with pyoderma in dogs supports the notion that the toxins are important for staphylococcal survival and pathogenesis. |
| Databáze: | OpenAIRE |
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