Internal heating method of loop-mediated isothermal amplification for detection of HPV-6 DNA

Autor:	Huan-Huan Zhu, Yuan Li, Li-Xia Wu, Ke-Sheng Wang, Yue Zhang, Qiang-Yuan Fan, Zun-Zhen Ming, Wei-Qin Chen, Wei-Wei Liu
Rok vydání:	2022
Předmět:	Heating Molecular Diagnostic Techniques DNA Human papillomavirus 6 Nucleic Acid Amplification Techniques Sensitivity and Specificity Analytical Chemistry
Zdroj:	Mikrochimica acta. 189(5)
ISSN:	1436-5073
Popis:	Loop-mediated isothermal amplification (LAMP) is a promising diagnostic tool for genetic amplification, which is known for its rapid process, simple operation, high amplification efficiency, and excellent sensitivity. However, most of the existing heating methods are external for completion of molecular amplification with possibility of contamination of specimens. The present research provided an internal heating method for LAMP using magnetic nanoparticles (MNPs), which is called nano-LAMP. Near-infrared light with an excitation wavelength of 808 nm was employed as the heating source; hydroxy naphthol blue (HNB) was used as an indicator to conduct methodological research. We demonstrate that the best temperature was controlled at a working power of 2 W and 4.8 µg/µL concentration of nanoparticles. The lowest limit for the detection of HPV by the nano-LAMP method is 10
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7e38da7de5bc65216fd701bf0c2bdeb4 https://pubmed.ncbi.nlm.nih.gov/35507110 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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