A New in vitro Assay System for Prolactin-Releasing Activity and its Application for Demonstration of Porcine Hypothalamic Prolactin-Releasing Factor (PRF)
| Autor: | Mitsuo Nomura, Kaoru Miyamoto, Masao Igarashi, Yoshito Ibuki, Yoshihisa Hasegawa |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
endocrine system
medicine.medical_specialty Swine Dopamine Ion chromatography Thyrotropin-releasing hormone Biology High-performance liquid chromatography Pituitary Gland Anterior Internal medicine Methods medicine Animals Thyrotropin-Releasing Hormone Bromocriptine Cells Cultured Obstetrics and Gynecology In vitro Prolactin Rats Endocrinology Cell culture Sephadex Female hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Asia-Oceania Journal of Obstetrics and Gynaecology. 11:271-278 |
| ISSN: | 0389-2328 |
| DOI: | 10.1111/j.1447-0756.1985.tb00745.x |
| Popis: | A new sensitive and reproducible assay system for prolactin-releasing activity (PRA) using a rat pituitary cell culture system was established. After pretreatment with bromocriptine (1.3 times 10-7 M) in culture medium for 24 hr on the 4th day in culture, the 6 hr addition of thyrotropin releasing hormone (TRH) to culture medium increased prolactin secretion in a dose-dependent manner at concentrations of TRH ranging from 3.2 times 10-11 M to 2.0 times 10-9 M; maximal prolactin response was 270% of the control value. By using this new assay system for PRA, prolactin-releasing factor (PRF) in porcine hypothalami was isolated and identified. On Sephadex G-25 gel-chromatography of acid extracts of porcine hypothalami, PRA was found in the small peptide portion. These fractions of PRA were further subjected to CM-cellulose (CM-52) ion exchange chromatography, followed by reverse-phase HPLC. PRA obtained by HPLC chromatography was neutralized by anti-TRH antibody. It was concluded that TRH is the main PRF in porcine hypothalamic extracts. |
| Databáze: | OpenAIRE |
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